TRMT1 Rekombinanter Antikörper

TRMT1 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

250178D5

Kat-Nr. : 85897-5-RR

Synonyme

EC:2.1.1.216, TRM1, TRMT 1, tRNA (guanine(26)-N(2))-dimethyltransferase, tRNA 2,2-dimethylguanosine-26 methyltransferase

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  WB analysis using 85897-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85897-5-RR (TRMT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 85897-5-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TRMT1 antibody (85897-5-RR, Clone: 250178D5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 85897-5-RR

  Biolayer interferometry (BLl) kinetic assays of 85897-5-RR against Human TRMT1 were performed. The affinity constant is 26.2 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HCT 116-Zellen, HEK-293T-Zellen, HepG2-Zellen, K-562-Zellen, Rattenhodengewebe
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85897-5-RR bindet in WB, IF/ICC, ELISA TRMT1 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TRMT1 fusion protein Ag6837
• Vollständiger Name: TRM1 tRNA methyltransferase 1 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 81 kDa
• Beobachtetes Molekulargewicht: 75 kDa
• GenBank-Zugangsnummer: BC002492
• Gene symbol: TRMT1
• Gene ID (NCBI): 55621
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRMT1 is an RNA methyltransferase responsible for the formation of N2,N2-dimethylguanosine (m2,2G) at position 26 in both cytosolic and mitochondrial tRNAs.TRMT1-catalyzed tRNA modifications are important for maintenance of global protein translation levels including proteins involved in cellular growth, development, and stress response. Human neural stem cells with TRMT1 knockdowns were found to have hypersensitivity to redox stress, implicating TRMT1 and the m2,2G26 modification in the regulation of redox homeostasis.