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TRMT10B Polyklonaler Antikörper

TRMT10B Polyklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 33056-1-PBS

Synonyme

EC:2.1.1.221, RG9MTD3, RNA (guanine-9-)-methyltransferase domain-containing protein 3, tRNA (guanine(9)-N(1))-methyltransferase TRMT10B, tRNA methyltransferase 10 homolog B

Filter:
  • Western Blot (WB) analysis of various lysates using TRMT10B Polyclonal antibody (33056-1-AP)

    WB analysis using 33056-1-AP (same clone as 33056-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 33056-1-AP (RG9MTD3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 33056-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

33056-1-PBS bindet in WB, Indirect ELISA TRMT10B und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen TRMT10B fusion protein Ag36835
Vollständiger Name RNA (guanine-9-) methyltransferase domain containing 3
Berechnetes Molekulargewicht36 kDa
Beobachtetes Molekulargewicht40 kDa
GenBank-ZugangsnummerBC012175
Gene symbol RG9MTD3
Gene ID (NCBI) 158234
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The TRM10 family of methyltransferases is responsible for the N1-methylation of purines at position 9 of tRNAs in Archaea and Eukarya. The human genome encodes three TRM10-type enzymes. TRMT10B is the first m1A9-specific tRNA methyltransferase found in eukaryotes and is responsible for the modification of a single nuclear-encoded tRNA. Furthermore, we show that the lack of G9 methylation causes a decrease in the steady-state levels of the initiator tRNAiMet-CAT and an alteration in its further post-transcriptional modification (PMID: 32392304).