TRMT10C Polyklonaler Antikörper

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TRMT10C Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 29087-1-AP

Synonyme

RG9MTD1, MRPP1

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  WB analysis using 29087-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29087-1-AP (TRMT10C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 29087-1-AP

  WB result of TRMT10C antibody (29087-1-AP; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRMT10C transfected HepG2 cells.

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  IHC staining of human colon cancer using 29087-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29087-1-AP (TRMT10C antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 29087-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29087-1-AP (TRMT10C antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 29087-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using TRMT10C antibody (29087-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, Mausherzgewebe, PC-12-Zellen, Rattenherzgewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:2000-1:8000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below

Produktinformation

29087-1-AP bindet in WB, IHC, IF/ICC, ELISA TRMT10C und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TRMT10C fusion protein Ag30642
• Vollständiger Name: RNA (guanine-9-) methyltransferase domain containing 1
• Berechnetes Molekulargewicht: 47 kDa
• Beobachtetes Molekulargewicht: 40-45 kDa
• GenBank-Zugangsnummer: NM_017819
• Gene symbol: TRMT10C
• Gene ID (NCBI): 54931
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

tRNA methyltransferase 10 homolog C (TRMT10C, also known as MRPP1) is involved in mitochondrial RNA processing and modification (PMID: 21593607). It is essential for the 5'-ends processing of mitochondrial tRNAs and plays a critical role in mitochondrial respiration and translation (PMID: 18984158; 29040705). Mutations in TRMT10C may be a cause of mitochondrial disease and highlight the importance of RNA processing for correct mitochondrial function (PMID: 27132592).

Publikationen

Species	Application	Title
human	WB	Cell Death Discov; N1-Methyladenosine modification of mRNA regulates neuronal gene expression and oxygen glucose deprivation/reoxygenation induction; Authors - Zhangyang Qi
mouse	WB	Circulation; Stimulates Short-Chain Dehydrogenase/Reductase Proteins to Alleviate Heart Failure Independent of Mitochondrial Protein Deacetylation; Authors - Matthew A Walker
mouse,human	WB	Phytother Res; Astragaloside IV protects renal tubular epithelial cells against oxidative stress-induced injury by upregulating CPT1A-mediated HSD17B10 lysine succinylation in diabetic kidney disease; Authors - Meng Wang
mouse	WB	BMC Cardiovasc Disord; Single-cell transcriptomics reveals writers of RNA modification-mediated immune microenvironment and cardiac resident Macro-MYL2 macrophages in heart failure; Authors - Yao-Lin Yang
	WB	Cancer Lett; YTHDF3 Drives Tumor Growth and Metastasis by Recruiting eIF4B to Promote Notch2 Translation in Breast Cancer; Authors - Hongyu Chen