TRMT10C Polyklonaler Antikörper

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• KD/KO Validated

TRMT10C Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29087-1-PBS

Synonyme

RG9MTD1, MRPP1

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  WB analysis using 29087-1-AP (same clone as 29087-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29087-1-AP (TRMT10C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29087-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 29087-1-AP (same clone as 29087-1-PBS)

  WB result of TRMT10C antibody (29087-1-AP; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRMT10C transfected HepG2 cells. This data was developed using the same antibody clone with 29087-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 29087-1-AP (same clone as 29087-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29087-1-AP (TRMT10C antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29087-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 29087-1-AP (same clone as 29087-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29087-1-AP (TRMT10C antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29087-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 29087-1-AP (same clone as 29087-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using TRMT10C antibody (29087-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 29087-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

29087-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA TRMT10C und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TRMT10C fusion protein Ag30642
• Vollständiger Name: RNA (guanine-9-) methyltransferase domain containing 1
• Berechnetes Molekulargewicht: 47 kDa
• Beobachtetes Molekulargewicht: 40-45 kDa
• GenBank-Zugangsnummer: NM_017819
• Gene symbol: TRMT10C
• Gene ID (NCBI): 54931
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

tRNA methyltransferase 10 homolog C (TRMT10C, also known as MRPP1) is involved in mitochondrial RNA processing and modification (PMID: 21593607). It is essential for the 5'-ends processing of mitochondrial tRNAs and plays a critical role in mitochondrial respiration and translation (PMID: 18984158; 29040705). Mutations in TRMT10C may be a cause of mitochondrial disease and highlight the importance of RNA processing for correct mitochondrial function (PMID: 27132592).