TRMT11 Polyklonaler Antikörper

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• KD/KO Validated

TRMT11 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 17555-1-AP

Synonyme

tRNA guanosine-2'-O-methyltransferase TRM11 homolog, tRNA (guanine(10)-N2)-methyltransferase homolog, TRM11, MDS024, EC:2.1.1.-

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  WB analysis of HEK-293 using 17555-1-AP

  WB result of TRMT11 antibody (17555-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRMT11 transfected HEK-293 cells.

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  WB analysis using 17555-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17555-1-AP (TRMT11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of LNCaP using 17555-1-AP

  LNCaP cells were subjected to SDS PAGE followed by western blot with 17555-1-AP (TRMT11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 17555-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17555-1-AP (TRMT11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 17555-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 17555-1-AP (TRMT11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 17555-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 17555-1-AP (TRMT11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 17555-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17555-1-AP (TRMT11 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 17555-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17555-1-AP (TRMT11 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 17555-1-AP

  Immunofluorescent analysis of HepG2 cells, using IFT52 antibody 17555-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, K-562-Zellen, LNCaP-Zellen, Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:300-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

17555-1-AP bindet in WB, IHC, IF/ICC, ELISA TRMT11 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TRMT11 fusion protein Ag11717
• Vollständiger Name: tRNA methyltransferase 11 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 463 aa, 53 kDa
• Beobachtetes Molekulargewicht: 48-53 kDa
• GenBank-Zugangsnummer: BC056878
• Gene symbol: TRMT11
• Gene ID (NCBI): 60487
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Microbiol Immunol; BUD23-TRMT112 interacts with the L protein of Borna disease virus and mediates the chromosomal tethering of viral ribonucleoproteins.; Authors - Bea Clarise B Garcia