TRMT2A Rekombinanter Antikörper

TRMT2A Rekombinant Antikörper für WB, IHC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240444C2

Kat-Nr. : 83463-8-PBS

Synonyme

tRNA (uracil 5 ) methyltransferase homolog A, mRNA, HTF9C, EC:2.1.1.35, EC:2.1.1.-

---

• 

  WB analysis using 83463-8-RR (same clone as 83463-8-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83463-8-RR (TRMT2A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83463-8-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 83463-8-RR (same clone as 83463-8-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83463-8-RR (TRMT2A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83463-8-PBS in a different storage buffer formulation.

• 

  FC experiment of U-251 using 83463-8-RR (same clone as 83463-8-PBS)

  1x10^6 U-251 cells were intracellularly stained with 0.25 ug TRMT2A Recombinant antibody (83463-8-RR, Clone:240444C2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83463-8-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83463-8-RR

  Biolayer interferometry (BLl) kinetic assays of 83463-8-RR against Human TRMT2A were performed. The affinity constant is 1.86 nM.

---

Geprüfte Anwendungen

Produktinformation

83463-8-PBS bindet in WB, IHC, FC (Intra), Indirect ELISA TRMT2A und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TRMT2A fusion protein Ag9112
• Vollständiger Name: TRM2 tRNA methyltransferase 2 homolog A (S. cerevisiae)
• Berechnetes Molekulargewicht: 625 aa, 69 kDa
• Beobachtetes Molekulargewicht: 42-44 kDa, 68-75 kDa
• GenBank-Zugangsnummer: BC013352
• Gene symbol: TRMT2A
• Gene ID (NCBI): 27037
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

tRNA methyltransferase 2 homolog A (TRMT2A), also known as HTF9C is a novel cell cycle regulated protein, as associated with aggressive clinical course. HTF9C harbors a putative RNA recognition motif (RRM) in the N-terminus and the conserved uracil-C5-methyltransferase domain of the TrmA family in the C-terminus. HTF9C antibody can detect two bands about 68 kDa and 40 kDa (PMID: 20307320).