TRMT2A Rekombinanter Antikörper

TRMT2A Rekombinant Antikörper für WB, IHC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

240444C2

Kat-Nr. : 83463-8-RR

Synonyme

tRNA (uracil 5 ) methyltransferase homolog A, mRNA, HTF9C, EC:2.1.1.35, EC:2.1.1.-

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  WB analysis using 83463-8-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83463-8-RR (TRMT2A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 83463-8-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83463-8-RR (TRMT2A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of U-251 using 83463-8-RR

  1x10^6 U-251 cells were intracellularly stained with 0.25 ug TRMT2A Recombinant antibody (83463-8-RR, Clone:240444C2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83463-8-RR

  Biolayer interferometry (BLl) kinetic assays of 83463-8-RR against Human TRMT2A were performed. The affinity constant is 1.86 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, A431-Zellen, HepG2-Zellen, MCF-7-Zellen, MOLT-4-Zellen
• Erfolgreiche Detektion in IHC: Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC (Intra): U-251-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83463-8-RR bindet in WB, IHC, FC (Intra), ELISA TRMT2A und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TRMT2A fusion protein Ag9112
• Vollständiger Name: TRM2 tRNA methyltransferase 2 homolog A (S. cerevisiae)
• Berechnetes Molekulargewicht: 625 aa, 69 kDa
• Beobachtetes Molekulargewicht: 42-44 kDa, 68-75 kDa
• GenBank-Zugangsnummer: BC013352
• Gene symbol: TRMT2A
• Gene ID (NCBI): 27037
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

tRNA methyltransferase 2 homolog A (TRMT2A), also known as HTF9C is a novel cell cycle regulated protein, as associated with aggressive clinical course. HTF9C harbors a putative RNA recognition motif (RRM) in the N-terminus and the conserved uracil-C5-methyltransferase domain of the TrmA family in the C-terminus. HTF9C antibody can detect two bands about 68 kDa and 40 kDa (PMID: 20307320).