TRMT5 Polyklonaler Antikörper

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• KD/KO Validated

TRMT5 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 18255-1-AP

Synonyme

KIAA1393, M1G methyltransferase, TRM5, TRMT5, tRNA [GM37] methyltransferase, tRNA methyltransferase 5

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  WB analysis of MCF-7 using 18255-1-AP

  WB result of TRMT5 antibody (18255-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRMT5 transfected MCF-7 cells.

• 

  WB analysis of Jurkat using 18255-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 18255-1-AP (TRMT5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 18255-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 18255-1-AP (TRMT5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 18255-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 18255-1-AP (TRMT5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, MCF-7-Zellen, Maushodengewebe, NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below

Produktinformation

18255-1-AP bindet in WB, ELISA TRMT5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TRMT5 fusion protein Ag13075
• Vollständiger Name: TRM5 tRNA methyltransferase 5 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 509 aa, 58 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: BC108284
• Gene symbol: TRMT5
• Gene ID (NCBI): 57570
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human,rat	WB	Front Mol Biosci; Integrated Strategies of Diverse Feature Selection Methods Identify Aging-Based Reliable Gene Signatures for Ischemic Cardiomyopathy.; Authors - Huafeng Song
human,mouse	WB	J Zhejiang Univ Sci B; Targeting TRMT5 suppresses hepatocellular carcinoma progression via inhibiting the HIF-1α pathways; Authors - Qiong Zhao ; KD Validated
human	WB	Cell; PAX translocations remodel mitochondrial metabolism through altered leucine usage in rhabdomyosarcoma; Authors - Bhargab Kalita