TRMT6 Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

TRMT6 Rekombinant Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

240911D3

Kat-Nr. : 83843-6-RR

Synonyme

240911D3, CGI 09, CGI-09, mRNA methyladenosine-N(1)-methyltransferase non-catalytic subunit TRM6, TRM6

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  WB analysis using 83843-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83843-6-RR (TRMT6 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 83843-6-RR

  WB result of TRMT6 antibody (83843-6-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRMT6 transfected HepG2 cells.

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  IHC staining of human ovarian cancer using 83843-6-RR

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83843-6-RR (TRMT6 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 83843-6-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using TRMT6 antibody (83843-6-RR, Clone: 240911D3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 83843-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83843-6-RR against Human TRMT6 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: U-251-Zellen, HepG2-Zellen, HuH-7-Zellen, Maushirngewebe, SMMC-7721-Zellen, U-87 MG-Zellen
• Erfolgreiche Detektion in IHC: human ovarian cancer; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunhistochemie (IHC): IHC : 1:600-1:2400
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83843-6-RR bindet in WB, IHC, IF/ICC, ELISA TRMT6 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TRMT6 fusion protein Ag10176
• Vollständiger Name: tRNA methyltransferase 6 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 56 kDa
• Beobachtetes Molekulargewicht: 55-60 kDa
• GenBank-Zugangsnummer: BC001262
• Gene symbol: TRMT6
• Gene ID (NCBI): 51605
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Transfer RNA (tRNA) modifications help regulate the efficiency of mRNA translation by maintaining the correct reading frames within mRNA. TRMT6 (tRNA methyltransferase 6 ), also known as GCD10 or TRM6, is a 497 amino acid protein that localizes to the nucleus and exists as a heterodimer with TRM61. Expressed in liver, brain, ovary and testis, TRMT6 functions as a substrate-binding subunit of tRNA and is thought to catalyze the formation of N(1)-methyladenine at position 58 in initiator methionyl-tRNA. TRMT6 exists as three alternatively spliced isoforms that, in response to DNA damage, may be phosphorylated by ATM or ATR.