TRMU Rekombinanter Antikörper

TRMU Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

250434A4

Kat-Nr. : 86095-1-RR

Synonyme

EC:2.8.1.14, Mitochondrial tRNA-specific 2-thiouridylase 1, MTO2, MTO2 homolog, MTU1

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  WB analysis using 86095-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86095-1-RR (TRMU antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 86095-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86095-1-RR against Human TRMU were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, A549-Zellen, HeLa-Zellen, MCF-7-Zellen, NIH/3T3-Zellen, Rattenherzgewebe, U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86095-1-RR bindet in WB, ELISA TRMU und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TRMU fusion protein Ag21930
• Vollständiger Name: tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase
• Berechnetes Molekulargewicht: 421 aa, 48 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC027991
• Gene symbol: TRNT1
• Gene ID (NCBI): 55687
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRMU (tRNA m5C methyltransferase) is a mitochondrial protein involved in the modification of mitochondrial tRNA. Specifically, it catalyzes the formation of 5-methylcytidine at position 34 (m5C34) in the anticodon stem-loop of mitochondrial tRNAs, which is crucial for maintaining the stability and function of these tRNAs. TRMU is a nuclear-encoded protein located in the mitochondrial matrix. It plays a vital role in mitochondrial translation and oxidative phosphorylation.TRMU is associated with several genetic disorders. Mutations in the TRMU gene can cause mitochondrial diseases, such as infantile hepatic failure syndrome. (PMID:23625533)