TRNAU1AP,SECP43 Polyklonaler Antikörper

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• KD/KO Validated

TRNAU1AP,SECP43 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 15053-1-AP

Synonyme

PRO1902, RP4 669K10.4, SECP43, TRNAU1AP, TRNAU1AP,SECP43, TRSPAP1

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  WB analysis using 15053-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15053-1-AP (TRNAU1AP,SECP43 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 15053-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15053-1-AP (TRNAU1AP,SECP43 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15053-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15053-1-AP (TRNAU1AP,SECP43 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 15053-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15053-1-AP (TRNAU1AP,SECP43 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 15053-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 15053-1-AP (TRNAU1AP,SECP43 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 15053-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 15053-1-AP (TRNAU1AP,SECP43 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human hepatocirrhosis using 15053-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 15053-1-AP (TRNAU1AP,SECP43 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 15053-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 15053-1-AP (TRNAU1AP,SECP43 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, COLO 320-Zellen, Jurkat-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberzirrhosegewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below

Produktinformation

15053-1-AP bindet in WB, IHC, ELISA TRNAU1AP,SECP43 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TRNAU1AP,SECP43 fusion protein Ag7036
• Vollständiger Name: tRNA selenocysteine 1 associated protein 1
• Berechnetes Molekulargewicht: 32 kDa
• Beobachtetes Molekulargewicht: 36 kDa, 21 kDa
• GenBank-Zugangsnummer: BC000680
• Gene symbol: SECP43
• Gene ID (NCBI): 54952
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human,mouse	WB	Biochem Biophys Res Commun; Knockdown of Trnau1ap inhibits the proliferation and migration of NIH3T3, JEG-3 and Bewo cells via the PI3K/Akt signaling pathway.; Authors - Xiaoyan Hu
mouse	WB	Biol Trace Elem Res; Selenium Deficiency Leads to Changes in Renal Fibrosis Marker Proteins and Wnt/β-Catenin Signaling Pathway Components.; Authors - Tingting Lin
mouse	WB	Biol Trace Elem Res; Selenium Deficiency-Induced Damage and Altered Expression of Mitochondrial Biogenesis Markers in the Kidneys of Mice.; Authors - Hehuan Lai
rat	WB	Mol Med Rep; Role of tRNA selenocysteine 1 associated protein 1 in the proliferation and apoptosis of cardiomyocyte‑like H9c2 cells.; Authors - Meng-Di Li ; KD Validated