TRNP1 Rekombinanter Antikörper

TRNP1 Rekombinant Antikörper für IHC, IF/ICC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IHC, IF/ICC, IF-P, ELISA

Konjugation

Unkonjugiert

CloneNo.

240331B6

Kat-Nr. : 83410-4-RR

Synonyme

TRNP, TNRP, TMF-regulated nuclear protein 1, C1orf225, 240331B6

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  IHC staining of mouse brain using 83410-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83410-4-RR (TRNP1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 83410-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83410-4-RR (TRNP1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 83410-4-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using TRNP1 antibody (83410-4-RR, Clone: 240331B6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 83410-4-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TRNP1 antibody (83410-4-RR, Clone: 240331B6 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of HeLa using 83410-4-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug TRNP1 Recombinant antibody (83410-4-RR, Clone:240331B6) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 83410-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83410-4-RR against Human TRNP1 were performed. The affinity constant is 2.5 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushirngewebe
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:125-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83410-4-RR bindet in IHC, IF/ICC, IF-P, ELISA TRNP1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TRNP1 fusion protein Ag22504
• Vollständiger Name: TMF1-regulated nuclear protein 1
• Berechnetes Molekulargewicht: 227 aa, 23 kDa
• GenBank-Zugangsnummer: BC069216
• Gene symbol: TRNP1
• Gene ID (NCBI): 388610
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRNP1 (TMF-regulated nuclear protein 1), also called C1orf225, is a DNA-binding factor that regulates the expression of a subset of genes and plays a key role in tangential, radial, and lateral expansion of the brain neocortex (PMID: 27264089; 23622239; 36947129). It has been shown to exert potent roles in neural development affecting neural stem cell self-renewal and brain folding (PMID: 32627867). It may also control the proliferation rate of cells by regulating their progression through key cell-cycle transition points.