TRPA1 Polyklonaler Antikörper

TRPA1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(31)

Kat-Nr. : 19124-1-AP

Synonyme

ANKTM1, p120, Wasabi receptor

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  WB analysis using 19124-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19124-1-AP (TRPA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 19124-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19124-1-AP (TRPA1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of rat dorsal root ganglion using 19124-1-AP

  Immunohistochemical analysis of paraffin-embedded rat dorsal root ganglion tissue slide using 19124-1-AP (TRPA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 19124-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 19124-1-AP (TRPA1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 19124-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 19124-1-AP (TRPA1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 19124-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19124-1-AP (TRPA1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 19124-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19124-1-AP (TRPA1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 19124-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19124-1-AP (TRPA1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SH-SY5Y-Zellen, Hausschwein-Kolongewebe
• Erfolgreiche Detektion in IHC: Maus-Cerebellum-Gewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 24 publications below
• IHC: See 6 publications below
• IF: See 10 publications below

Produktinformation

19124-1-AP bindet in WB, IHC, IF, ELISA TRPA1 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: transient receptor potential cation channel, subfamily A, member 1
• Berechnetes Molekulargewicht: 140 kDa
• Beobachtetes Molekulargewicht: 120-130 kDa
• GenBank-Zugangsnummer: NM_007332
• Gene symbol: TRPA1
• Gene ID (NCBI): 8989
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRPA1, also named as ANKTM1, belongs to the transient receptor family. TRPA1 is a receptor-activated non-selective cation channel involved in detection of pain and possibly also in cold perception and inner ear function. TRPA1 has a central role in the pain response to endogenous inflammatory mediators and to a diverse array of volatile irritants, such as mustard oil, garlic and acrolein, an irritant from tears gas and vehicule exhaust fumes. It acts also as a ionotropic cannabinoid receptor by being activated by delta(9)-tetrahydrocannabinol (THC), the psychoactive component of marijuana. It may be a component for the mechanosensitive transduction channel of hair cells in inner ear, thereby participating in the perception of sounds.

Publikationen

Species	Application	Title
mouse	WB,IF	Metabolism; IFT80 and TRPA1 cooperatively regulate bone formation by calcium signaling in response to mechanical stimuli; Authors - Ting Wang
rat	WB	Phytomedicine; Yu-Xue-Bi capsule ameliorates aggressive synovitis and joint damage in rheumatoid arthritis via modulating the SUCNR1/HIF-1α/TRPV1 axis; Authors - Wenjia Chen
mouse	IHC	J Control Release; A dual receptors-targeting and size-switchable "cluster bomb" co-loading chemotherapeutic and transient receptor potential ankyrin 1 (TRPA-1) inhibitor for treatment of triple negative breast cancer.; Authors - Yashi Wang
		Front Pharmacol; The efficacy of ophiopogonanone B in treating the cough in mice infected with Mycoplasma pneumoniae; Authors - Nan-Nan Liu
rat,human	WB,IF	J Ethnopharmacol; Ligustilide covalently binds to Cys703 in the pre-S1 helix of TRPA1, blocking the opening of channel and relieving pain in rats with acute soft tissue injury; Authors - Kaixue Zhang
mouse	WB,IF	Mol Nutr Food Res; Menthol from Mentha piperita Suppresses the Milk Production of Lactating Mammary Epithelial Cells In Vivo and In Vitro.; Authors - Norihiro Suzuki