TRPC4 Polyklonaler Antikörper

TRPC4 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 21349-1-AP

Synonyme

Trp-related protein 4, TRP4, Trp related protein 4, hTrp-4, HTRP4

---

• 

  WB analysis using 21349-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21349-1-AP (TRPC4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 21349-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 21349-1-AP (TRPC4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 21349-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 21349-1-AP (TRPC4 antibody) at dilution of 1:50 (under 40x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

21349-1-AP bindet in WB, IHC, ELISA TRPC4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TRPC4 fusion protein Ag15698
• Vollständiger Name: transient receptor potential cation channel, subfamily C, member 4
• Berechnetes Molekulargewicht: 977 aa, 112 kDa
• Beobachtetes Molekulargewicht: 100-120 kDa
• GenBank-Zugangsnummer: BC104725
• Gene symbol: TRPC4
• Gene ID (NCBI): 7223
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRPC4 (Transient Receptor Potential Paradigm 4) is a non-selective calcium channel protein widely found in the nervous system and cardiovascular system. TRPC4 regulates intracellular calcium levels through the activation of signaling pathways mediated by Gq/11 and Gi/o-coupled receptors, and is involved in neurotransmission, neuronal excitability, and vascular endothelial cell function. In the nervous system, TRPC4 is associated with neuropsychiatric disorders such as depression and anxiety, and its inhibitors show antidepressant and anxiolytic potential. In the cardiovascular system, TRPC4 affects vascular permeability and diastolic function by regulating calcium ion inward flow and plays an important role in hypoxia-induced pulmonary hypertension. In addition, TRPC4 is involved in thermoregulation, sensing changes in internal temperature to regulate the drop in body temperature. aberrant expression of TRPC4 is associated with a variety of diseases, making it a potential therapeutic target!

Publikationen

Species	Application	Title
human		Sci Adv; Cryo-EM structure of TRPC5 at 2.8-Å resolution reveals unique and conserved structural elements essential for channel function.; Authors - Jingjing Duan
human	WB	Cancer Lett; TRPC3 promotes tumorigenesis of gastric cancer via the CNB2/GSK3β/NFATc2 signaling pathway.; Authors - Da-Cen Lin