TRPC4AP Monoklonaler Antikörper

TRPC4AP Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG3

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

2C10B1

Kat-Nr. : 66138-1-Ig

Synonyme

C20orf188, Protein TAP1, Protein TRUSS, Trp4 associated protein, Trpc4 associated protein, TRPC4AP, TRRP4AP, TRUSS

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  WB analysis of human brain using 66138-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 66138-1-Ig (TRPC4AP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 66138-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 66138-1-Ig (TRPC4AP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 66138-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver using 66138-1-Ig(TRPC4AP antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 66138-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 66138-1-Ig (TRPC4AP Antibody) at dilution of 1:50.

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  IHC staining of human liver using 66138-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver using 66138-1-Ig(TRPC4AP antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 66138-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 66138-1-Ig (TRPC4AP Antibody) at dilution of 1:50.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, humanes Herzgewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

66138-1-Ig bindet in WB, IHC, ELISA TRPC4AP und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Maus / IgG3
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TRPC4AP fusion protein Ag18772
• Vollständiger Name: transient receptor potential cation channel, subfamily C, member 4 associated protein
• Berechnetes Molekulargewicht: 797 aa, 91 kDa
• Beobachtetes Molekulargewicht: 82 kDa
• GenBank-Zugangsnummer: BC013144
• Gene symbol: TRPC4AP
• Gene ID (NCBI): 26133
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRPC4AP, also named as C20orf188 and TRRP4AP, is a substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control. TRPC4AP participates in the activation of NFKB1 in response to ligation of TNFRSF1A, possibly by linking TNFRSF1A to the IKK signalosome. The calculated molecular weight of TRPC4AP is 91 kDa. Western blot analysis detected a protein of about 82 kDa expressed in several mouse cell lines and in a human promonocytic cell line.

Publikationen

Species	Application	Title
mouse	WB	Nat Commun; A glucose-enriched lung pre-metastatic niche triggered by matrix stiffness-tuned exosomal miRNAs in hepatocellular carcinoma; Authors - Yingying Zhao