TRPM4 Polyklonaler Antikörper

TRPM4 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21985-1-PBS

Synonyme

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  WB analysis using 21985-1-AP (same clone as 21985-1-PBS)

  HEK-293 cells( total protein heated at 100℃, membrane protein incubated at 37℃) were subjected to SDS PAGE followed by western blot with 21985-1-AP (TRPM4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21985-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 21985-1-AP (same clone as 21985-1-PBS)

  HEK-293 cells lysates (total protein heated at 100℃, total protein incubated at 37℃) were subjected to SDS PAGE followed by western blot with 21985-1-AP (TRPM4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21985-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 21985-1-AP (same clone as 21985-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21985-1-AP (TRPM4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21985-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 21985-1-AP (same clone as 21985-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21985-1-AP (TRPM4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21985-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

21985-1-PBS bindet in WB, IHC, Indirect ELISA TRPM4 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TRPM4 fusion protein Ag17132
• Vollständiger Name: transient receptor potential cation channel, subfamily M, member 4
• Berechnetes Molekulargewicht: 1214 aa, 134 kDa
• Beobachtetes Molekulargewicht: 130~150 kDa
• GenBank-Zugangsnummer: BC132727
• Gene symbol: TRPM4
• Gene ID (NCBI): 54795
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRPM4 is a Calcium-activated nonselective cation channel. It is activated by increase in intracellular Ca2+, mediates transport of monovalent cations (Na+ > K+ > Cs+ > Li+), leading to depolarize the membrane. It thereby plays a central role in cadiomyocytes, neurons from entorhinal cortex, dorsal root and vomeronasal neurons, endocrine pancreas cells, kidney epithelial cells, cochlea hair cells etc. TRPM4 participates in T-cell activation by modulating Ca2+ oscillations after T lymphocyte activation, which is required for NFAT-dependent IL2 production. It's also involved in myogenic constriction of cerebral arteries.