TRPM5 Polyklonaler Antikörper

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• KD/KO Validated

TRPM5 Polyklonal Antikörper für WB, IHC, IF, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(10)

Kat-Nr. : 18027-1-AP

Synonyme

LTrpC 5, LTrpC5, LTrpC-5, MLSN1- and TRP-related gene 1 protein, MTR1

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  WB analysis of mouse liver using 18027-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18027-1-AP (TRPM5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human small intestine using 18027-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 18027-1-AP (TRPM5 antibody) at dilution of 1:200 (under 10x lens)..

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  IHC staining of human small intestine using 18027-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 18027-1-AP (TRPM5 antibody) at dilution of 1:200 (under 40x lens)..

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  IF Staining of mouse olfactory epithelium tissue using 18027-1-AP

  Fluorescent IHC on 1%PLP fixed frozen mouse olfactory epithelium tissue of TRPM5 antibody (18027-1-AP, 1:200). Microvillar cell staining in the apical layer. By Dr. Brian Lin (Schwob Lab).

• 

  FC experiment of LNCaP using 18027-1-AP

  1x10^6 LNCaP cells were intracellularly stained with 0.4 ug TRPM5 Polyclonal antibody (18027-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Dünndarmgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maus-Riechepithelgewebe
• Erfolgreiche Detektion in FC (Intra): LNCaP-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:200
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 5 publications below

Produktinformation

18027-1-AP bindet in WB, IHC, IF, FC (Intra), ELISA TRPM5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TRPM5 fusion protein Ag12593
• Vollständiger Name: transient receptor potential cation channel, subfamily M, member 5
• Berechnetes Molekulargewicht: 98 kDa, 131 kDa
• Beobachtetes Molekulargewicht: 98 kDa
• GenBank-Zugangsnummer: BC093787
• Gene symbol: TRPM5
• Gene ID (NCBI): 29850
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Transient receptor potential (TRP) proteins are a diverse family of proteins with structural features typical of ion channels (PMID: 14634208). TRPM5 is a member of the TRPM (melastatin-like) subfamily which are Ca(2+)-permeable cation channels localized predominantly to the plasma membrane (PMID: 11864597). TRPM5 plays a central role in taste transduction (PMID: 17610722). TRPM5 is implicated in enhancing TRPA1 expression and may be involved in regulating insulin secretion (PMID: 21932052). Alternative splicing results in transcript variants encoding distinct isoforms with calculated molecular weights of 98 kDa or 131 kDa. It has been reported that TRPM5 is N-linked glycosylated at a unique site and TRPM5 glycosylation seems not to be involved in channel trafficking, but mainly in its functional regulation (PMID: 24605085).

Publikationen

Species	Application	Title
mouse	WB	Immunity; Intestinal Tuft-2 cells exert antimicrobial immunity via sensing bacterial metabolite N-undecanoylglycine.; Authors - Zhen Xiong
human		Food Funct; Piperine, as a TAS2R14 agonist, stimulates the secretion of glucagon-like peptide-1 in the human enteroendocrine cell line Caco-2.; Authors - Ting-Ting Huang ; KD Validated
rat	IF	Appetite; Chronic exposure to liquid sucrose and dry sucrose diet have differential effects on peripheral taste responses in female rats.; Authors - Lynnette Phillips McCluskey
human	IF	Heliyon; Involvement of pore helix in voltage-dependent inactivation of TRPM5 channel.; Authors - Kunitoshi Uchida
mouse,human	WB	Respir Res; Human airway tuft cells influence the mucociliary clearance through cholinergic signalling; Authors - Monika I Hollenhorst
mouse	WB,IF	Curr Med Sci; Insufficient TRPM5 Mediates Lipotoxicity-induced Pancreatic β-cell Dysfunction; Authors - Kai-Yuan Wang