TRPS1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TRPS1 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Kat-Nr. : 20003-1-AP

Synonyme

GC79, LGCR, TRPS1, Zinc finger protein GC79

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 20003-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20003-1-AP (TRPS1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 20003-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20003-1-AP (TRPS1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 20003-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 20003-1-AP (TRPS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 20003-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 20003-1-AP (TRPS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 20003-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20003-1-AP (TRPS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa cells using 20003-1-AP

  IP result of anti-TRPS1 (20003-1-AP for IP and Detection).

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  IHC staining of human prostate cancer using 20003-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer slide using 20003-1-AP (TRPS1 Antibody) at dilution of 1:50.

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  IHC staining of human prostate cancer using 20003-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer slide using 20003-1-AP (TRPS1 Antibody) at dilution of 1:50.

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  IHC staining of mouse liver using 20003-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 20003-1-AP (TRPS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 20003-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 20003-1-AP (TRPS1 Antibody) at dilution of 1:50.

• 

  IHC staining of human kidney using 20003-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 20003-1-AP (TRPS1 Antibody) at dilution of 1:50.

• 

  IHC staining of mouse brain using 20003-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20003-1-AP (TRPS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 20003-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20003-1-AP (TRPS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 20003-1-AP

  Immunofluorescent analysis of HepG2 cells, using TRPS1 antibody 20003-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Mausnierengewebe, Rattenhirngewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, humanes Nierengewebe, Maushirngewebe, Mauslebergewebe, Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 5 publications below
• IHC: See 4 publications below
• IF: See 3 publications below
• ChIP: See 3 publications below

Produktinformation

20003-1-AP bindet in WB, IP, IHC, IF, ChIP, ELISA TRPS1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: trichorhinophalangeal syndrome I
• Berechnetes Molekulargewicht: 142 kDa
• Beobachtetes Molekulargewicht: 135-142 kDa
• GenBank-Zugangsnummer: NM_014112
• Gene symbol: TRPS1
• Gene ID (NCBI): 7227
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRPS1, also named as GC79 and LGCR, is a transcriptional repressor. TRPS1 may act to restrict expression of GATA-regulated genes at selected sites and stages in vertebrate development. It might be involved in prostate cancer apoptosis. Defects in TRPS1 are the cause of tricho-rhino-phalangeal syndrome type 1/2/3 (TRPS1) (TRPS2) (TRPS3). The antibody recognizes the C-term of TRPS1.

Publikationen

Species	Application	Title
mouse	IF	Cell Stem Cell; Multi-omic analysis reveals divergent molecular events in scarring and regenerative wound healing.; Authors - Shamik Mascharak
human	WB	J Pathol; Down-regulation of TRPS1 stimulates epithelial-mesenchymal transition and metastasis through repression of FOXA1.; Authors - Jin-Zhou Huang ; KD Validated
human	IHC	Int J Mol Med; Pro‑differentiating compounds for human intervertebral disc cells are present in Violina pumpkin leaf extracts; Authors - Elisabetta Lambertini
	WB	J Biol Chem; Dual Role of the Trps1 Transcription Factor in Dentin Mineralization.; Authors - Maria Kuzynski ; KD Validated
human	IHC,ChIP	J Cell Physiol; Expression and function of the P2X7 receptor in human osteoblasts: The role of NFATc1 transcription factor.; Authors - Leticia Scussel Bergamin
human	IHC,IF	Life Sci; Decellularized extracellular matrix-based scaffold and hypoxic priming: A promising combination to improve the phenotype of degenerate intervertebral disc cells.; Authors - Letizia Penolazzi