TRPS1 Polyklonaler Antikörper

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• KD/KO Validated

TRPS1 Polyklonal Antikörper für WB, IF/ICC, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IF-P, IP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 21938-1-AP

Synonyme

GC79, LGCR, Tricho-rhino-phalangeal syndrome type I protein

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  WB analysis using 21938-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21938-1-AP (TRPS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 21938-1-AP

  WB result of TRPS1 antibody (21938-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRPS1 transfected HEK-293 cells.

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  WB analysis of HeLa using 21938-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21938-1-AP (TRPS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 21938-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21938-1-AP (TRPS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse lung using 21938-1-AP

  IP result of anti-TRPS1 (IP:21938-1-AP, 4ug; Detection:21938-1-AP 1:1000) with mouse lung tissue lysate 3600ug.

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  IF Staining of mouse skin using 21938-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skin tissue using TRPS1 antibody (21938-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse skin using 21938-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skin tissue using TRPS1 antibody (21938-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of MCF-7 using 21938-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using TRPS1 antibody (21938-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), (CL488-Phalloidin, green).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, Mausnierengewebe, Mauslungengewebe, Maushodengewebe
• Erfolgreiche IP: Mauslungengewebe
• Erfolgreiche Detektion in IF-P: Maushautgewebe
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 2 publications below
• ChIP: See 1 publications below

Produktinformation

21938-1-AP bindet in WB, IF/ICC, IF-P, IP, ChIP, ELISA TRPS1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TRPS1 fusion protein Ag16583
• Vollständiger Name: trichorhinophalangeal syndrome I
• Berechnetes Molekulargewicht: 1294 aa, 143 kDa
• Beobachtetes Molekulargewicht: 150 kDa
• GenBank-Zugangsnummer: BC125020
• Gene symbol: TRPS1
• Gene ID (NCBI): 7227
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRPS1 is a zinc finger transcriptional repressor involved in the regulation of chondrocyte and perichondrium development, containing a GATA-type zinc finger through which it binds to DNA. with nine zinc-finger domains and two C-terminal Ikaros-like zinc fingers. Its reperssible function was dependent on the integrity of the Trps1 GATA-type zinc-finger domain and also required the C-terminal 119 amino acids of the protein, which harbor the two Ikaros-like zinc-finger domains

Publikationen

Species	Application	Title
human	WB	Cell Death Discov; The feedback loop between miR-222-3p and ZEB1 harnesses metastasis in renal cell carcinoma; Authors - Fan Wang
human	WB,ChIP	Cell Biosci; Tie2-expressing monocytes/macrophages promote angiogenesis in chronically ischaemic brain tissue; Authors - Chuyang Tai
human		Biochim Biophys Acta Mol Basis Dis; UVB-induced TRPS1 regulates MITF transcription activity to promote skin pigmentation; Authors - Yushan Zhang
mouse	IF	Acta Biomater; Shape-Fixing Hydrogel Promotes Scarless Healing of Wounds Under Tension; Authors - Danlan Fu
human	IF	Clin Transl Oncol; Endoplasmic reticulum stress regulates apoptosis and chemotherapeutic via enhancing TNFRSF10B recycling to the cell membrane in triple-negative breast cancer; Authors - Dapeng Zhao