Tested Applications
| Positive WB detected in | HepG2 cells, MDA-MB-468 cells, T-47D cells, HEK-293 cells, Y79 cells, HUVEC cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66983-1-Ig targets TRPV1 in WB, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, sheep |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18683 Product name: Recombinant human TRPV1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-203 aa of BC132820 Sequence: MKKWSSTDLGAAADPLQKDTCPDPLDGDPNSRPPPAKPQLSTAKSRTRLFGKGDSEEAFPVDCPHEEGELDSCPTITVSPVITIQRPGDGPTGARLLSQDSVAASTEKTLRLYDRRSIFEAVAQNNCQDLESLLLFLQKSKKHLTDNEFKDPETGKTCLLKAMLNLHDGQNTTIPLLLEIARQTDSLKELVNASYTDSYYKGQ Predict reactive species |
| Full Name | transient receptor potential cation channel, subfamily V, member 1 |
| Calculated Molecular Weight | 839 aa, 95 kDa |
| Observed Molecular Weight | 105 kDa |
| GenBank Accession Number | BC132820 |
| Gene Symbol | TRPV1 |
| Gene ID (NCBI) | 7442 |
| RRID | AB_2882302 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q8NER1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TRPV1, also named as VR1 or OTRPC1, belongs to the transient receptor potential (TRP) family and TRPV subfamily (PMID: 17579562). TRPV1 is a ligand-activated non-selective calcium permeant cation channel expressed predominantly by sensory neurons (PMID: 10821274). It is involved in detection of noxious chemical and thermal stimuli. TRPV1 plays an important role in inflammatory thermal hyperalgesia (PMID: 10764638; 10821274).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TRPV1 antibody 66983-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
TRPV1 antibody (Cat# 66983-1-Ig) has 71 citations across 50+ journals including Nature Communications, PNAS, Cell Death & Disease, Phytomedicine, Journal of Ethnopharmacology, FASEB Journal, International Immunopharmacology, Frontiers in Pharmacology, and Molecular Pain. Research fields span pain neuroscience, inflammation, cancer, osteoarthritis, respiratory disease, liver/kidney injury, obesity, cardiovascular protection, and pharmacology/natural product research.
| Species | Application | Title |
|---|---|---|
Bioact Mater Manganese oxide nanozyme-loaded CO-releasing halloysite nanotubes for remodeling colonic immune, microbiota, and intestinal barrier homeostasis with concurrent pain relief in ulcerative colitis. | ||
Nat Commun Nanoparticle-mediated TRPV1 channel blockade amplifies cancer thermo-immunotherapy via heat shock factor 1 modulation | ||
Adv Sci (Weinh) Near Infrared Responsive Gold Nanorods Attenuate Osteoarthritis Progression by Targeting TRPV1 | ||
Research (Wash D C) Bifunctional TRPV1 Targeted Magnetothermal Switch to Attenuate Osteoarthritis Progression | ||
J Control Release Ultrasound-responsive piezoelectric analgesic microspheres alleviate osteoarthritis pain. | ||
Cell Death Dis TRPV1 alleviates osteoarthritis by inhibiting M1 macrophage polarization via Ca2+/CaMKII/Nrf2 signaling pathway. |
Reviews
What customers say
One reviewer (Shihab S.) tested this TRPV1 antibody by immunofluorescence at 1:100 dilution on a cell type known to express TRPV1, but was unable to detect any signal despite multiple attempts. The reviewer noted that they have had similar difficulties with other TRPV1 antibodies in this particular tissue type, so the lack of signal may not reflect poorly on the antibody itself. They planned to test it on other cell/tissue types to further evaluate its staining capabilities.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Shihab (Verified Customer) (09-17-2021) | This antibody was tried in a cell type (cannot disclose) which has previously been shown to express plenty of TRPV1 signal. However, this antibody was unable to give any signal in various attempts at staining, even at 1:100 dilution. This is not to say that this anybody is not good because I have had trouble with various TRPV1 antibodies in this tissue type but it will be used in other cell/tissue type to see its staining capabilities
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