TRPV4 Rekombinanter Antikörper

TRPV4 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

241143H1

Kat-Nr. : 84091-4-RR

Synonyme

Vanilloid receptor-like channel 2, TRP12, OTRPC4, Osm-9-like TRP channel 4, Osm 9 like TRP channel 4

---

• 

  WB analysis using 84091-4-RR

  Rat brain tissue(heated at 100 ° C and unboilded)lysates were subjected to SDS PAGE followed by western blot with 84091-4-RR (TRPV4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 84091-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84091-4-RR against Human TRPV4 were performed. The affinity constant is 2.57 nM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: unboiled rat brain tissue

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84091-4-RR bindet in WB, ELISA TRPV4 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TRPV4 fusion protein Ag27253
• Vollständiger Name: transient receptor potential cation channel, subfamily V, member 4
• Berechnetes Molekulargewicht: 98 kDa
• Beobachtetes Molekulargewicht: 85~98 kDa
• GenBank-Zugangsnummer: NM_001177428.1
• Gene symbol: TRPV4
• Gene ID (NCBI): 59341
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRPV4, also called VRL2 and VROAC, belongs to the transient receptor family and TrpV subfamily. It is a non-selective calcium permeant cation channel protein which involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. It is activated by low pH, citrate and phorbol esters. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism. Defects in TRPV4 are the cause of brachyolmia type 3 (BRAC3), spondylometaphyseal dysplasia Kozlowski type (SMDK) and metatropic dysplasia (MTD). Multiple transcript variants encoding different isoforms have been found.