TRPV6 Polyklonaler Antikörper

TRPV6 Polyklonal Antikörper für WB, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13411-1-PBS

Synonyme

CATL, CaT1, CaT like, CaT L, Calcium transport protein 1

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  WB analysis of human placenta using 13411-1-AP (same clone as 13411-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 13411-1-AP (TRPV6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13411-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 13411-1-AP (same clone as 13411-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 13411-1-AP (TRPV6 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13411-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse kidney using 13411-1-AP (same clone as 13411-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13411-1-AP (TRPV6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13411-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-3 using 13411-1-AP (same clone as 13411-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13411-1-AP (TRPV6 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13411-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse kidney using 13411-1-AP (same clone as 13411-1-PBS)

  IP result of anti-TRPV6 (IP:13411-1-AP, 4ug; Detection:13411-1-AP 1:300) with mouse kidney tissue lysate 4000ug. This data was developed using the same antibody clone with 13411-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 13411-1-AP (same clone as 13411-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TRPV6 antibody (13411-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 13411-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

13411-1-PBS bindet in WB, IF/ICC, IP, Indirect ELISA TRPV6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TRPV6 fusion protein Ag4060
• Vollständiger Name: transient receptor potential cation channel, subfamily V, member 6
• Berechnetes Molekulargewicht: 725 aa, 83 kDa
• Beobachtetes Molekulargewicht: 83 kDa, 70 kDa
• GenBank-Zugangsnummer: BC034814
• Gene symbol: TRPV6
• Gene ID (NCBI): 55503
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRPV6, also named as CAT1 or ECaC2, is a member of the transient receptor potential (TRP) family of membrane proteins. Unlike most TRP channels, TRPV6 and its closest relative, TRPV5, are calcium-selective channels. TRPV6 is highly expressed in the proximal intestine, placenta and exocrine tissues (PMID: 12869611). It is probably involved in calcium absorption in various tissues, including calcium reabsorption in kidney. TRPV6 is overexpressed in some cancers and exhibits oncogenic potential.