TRX2/TXN2 Rekombinanter Antikörper

TRX2/TXN2 Rekombinant Antikörper für WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Affe, human, Maus

Anwendung

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242707H4

Kat-Nr. : 85212-2-PBS

Synonyme

TRX2, MT TRX, MTRX, Mt-Trx, Thioredoxin 2

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  WB analysis using 85212-2-RR (same clone as 85212-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85212-2-RR (TRX2/TXN2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85212-2-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA sample test of MP01917-1

  The mean TRX2/TXN2 concentration was determined to be 1,925.24 ng/mL in Raji cell extract based on a 3.00 mg/mL extract load.

• 

  Sandwich ELISA standard curve of MP01917-1

  Sandwich ELISA standard curve of MP01917-1, Human TRX2/TXN2 Recombinant Matched Antibody Pair - PBS only. 85212-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag3640. 85212-1-PBS was HRP conjugated as the detection antibody. Range: 3.13-200 ng/mL.

• 

  Cytometric bead array standard curve of MP01917-1

  Cytometric bead array standard curve of MP01917-1, TRX2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85212-2-PBS. Detection antibody: 85212-1-PBS. Standard: Ag3640. Range: 0.781-100 ng/mL.

• 

  Affinity and Kinetic Characterization of 85212-2-RR

  Biolayer interferometry (BLl) kinetic assays of 85212-2-RR against Human TRX2/TXN2 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

85212-2-PBS bindet in WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA TRX2/TXN2 und zeigt Reaktivität mit Affe, human, Maus

• Getestete Reaktivität: Affe, human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TRX2/TXN2 fusion protein Ag3640
• Vollständiger Name: thioredoxin 2
• Berechnetes Molekulargewicht: 166 aa, 18 kDa
• Beobachtetes Molekulargewicht: 12 kDa
• GenBank-Zugangsnummer: BC013726
• Gene symbol: Thioredoxin 2
• Gene ID (NCBI): 25828
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TXN2 (Thioredoxin-2) is also named as TRX2, MTRX, Mt-TRX, and regulates the mitochondrial redox state and plays an important role in cell proliferation. It has been shown that cells conditionally deficient in Trx2 undergo apoptosis in the absence of exogenous stress, accompanied by an accumulation of intracellular ROS, the activation of caspase 9 and caspase 3, and the release of cytochrome c into the cytosol. (PMID: 18441302).