TRX2/TXN2 Rekombinanter Antikörper

TRX2/TXN2 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Affe, human, Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

242707H4

Kat-Nr. : 85212-2-RR

Synonyme

TRX2, MT TRX, MTRX, Mt-Trx, Thioredoxin 2

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  WB analysis using 85212-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85212-2-RR (TRX2/TXN2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 85212-2-RR

  Biolayer interferometry (BLl) kinetic assays of 85212-2-RR against Human TRX2/TXN2 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, C2C12-Zellen, COS-7-Zellen, HEK-293-Zellen, L02-Zellen, Raji-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85212-2-RR bindet in WB, ELISA TRX2/TXN2 und zeigt Reaktivität mit Affe, human, Maus

• Getestete Reaktivität: Affe, human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TRX2/TXN2 fusion protein Ag3640
• Vollständiger Name: thioredoxin 2
• Berechnetes Molekulargewicht: 166 aa, 18 kDa
• Beobachtetes Molekulargewicht: 12 kDa
• GenBank-Zugangsnummer: BC013726
• Gene symbol: Thioredoxin 2
• Gene ID (NCBI): 25828
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TXN2 (Thioredoxin-2) is also named as TRX2, MTRX, Mt-TRX, and regulates the mitochondrial redox state and plays an important role in cell proliferation. It has been shown that cells conditionally deficient in Trx2 undergo apoptosis in the absence of exogenous stress, accompanied by an accumulation of intracellular ROS, the activation of caspase 9 and caspase 3, and the release of cytochrome c into the cytosol. (PMID: 18441302).