Hamartin/TSC1 Polyklonaler Antikörper

Hamartin/TSC1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Rezensionen (2)

Kat-Nr. : 29906-1-AP

Synonyme

TSC1, TSC 1, TSC, LAM, KIAA0243

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  WB analysis of mouse kidney using 29906-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 29906-1-AP (Hamartin/TSC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 29906-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29906-1-AP (TSC1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 29906-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 29906-1-AP (Hamartin/TSC1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 29906-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 29906-1-AP (Hamartin/TSC1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 29906-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 29906-1-AP (Hamartin/TSC1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 29906-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 29906-1-AP (Hamartin/TSC1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of hTERT-RPE1 using 29906-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using Hamartin/TSC1 antibody (29906-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, MCF-7-Zellen, Mausnierengewebe
• Erfolgreiche Detektion in IHC: Mausnierengewebe, Maus-Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: hTERT-RPE1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

29906-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA Hamartin/TSC1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Hamartin/TSC1 fusion protein Ag31621
• Vollständiger Name: tuberous sclerosis 1
• Berechnetes Molekulargewicht: 130KD
• Beobachtetes Molekulargewicht: 140-145 kDa
• GenBank-Zugangsnummer: NM_000368
• Gene symbol: Hamartin/TSC1
• Gene ID (NCBI): 7248
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TSC1, also named as hamartin, interacts with TSC2 to form a protein complex that inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling (PubMed: 12271141, PubMed: 28215400). It can recruit TSC2 to HSP90AA1 and stabilizes TSC2 by preventing the interaction between TSC2 and ubiquitin ligase HERC1 (PubMed: 16464865, PubMed: 29127155). The loss of TSC1/TSC2 in mouse neurons resulted in a block in oligodendrocyte hypomyelination in vivo (PubMed: 28183733).

Publikationen

Species	Application	Title
mouse	IHC	Genes Dis; Naked cuticle homolog 2 controls the differentiation of osteoblasts and osteoclasts and ameliorates bone loss in ovariectomized mice; Authors - Liying Shan
human	WB	Mol Cell; YTHDF1 differentiates the contributing roles of mTORC1 in aging; Authors - Chenzhong Xu
human	WB	Elife; A cleaved METTL3 potentiates the METTL3-WTAP interaction and breast cancer progression; Authors - Chaojun Yan
human	WB	Cell Signal; METTL14-mediated N6-methyladenosine modification of TCP1 mRNA promotes acute myeloid leukemia progression; Authors - Mengmeng Zhang
human	WB,IP,IHC,IF	Br J Cancer; CDK4/6 inhibitors dephosphorylate RNF26 to stabilize TSC1 and increase the sensitivity of ccRCC to mTOR inhibitors; Authors - Xinlin Liu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Shivali (Verified Customer) (09-13-2025)

We are working on how negative regulators of mTOR complex affect translation and used this antibody to check TSC1 knockout cell lines. Worked well

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000

FH

Parijat (Verified Customer) (08-27-2025)

I used this antibody to confirm TSC2 knockout in both human (eHap1) and mouse (IMCD3) cells and it worked great. Although in Mouse there were few non-specific bands, it was much cleaner in Human cells.

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000