Hamartin/TSC1 Polyklonaler Antikörper

Hamartin/TSC1 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 29906-1-AP

Synonyme

Hamartin, KIAA0243, LAM, TSC, TSC1, tuberous sclerosis 1, Tuberous sclerosis 1 protein

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Galerie der Validierungsdaten

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  WB analysis of mouse kidney using 29906-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 29906-1-AP (Hamartin/TSC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 29906-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29906-1-AP (TSC1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 29906-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 29906-1-AP (Hamartin/TSC1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 29906-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 29906-1-AP (Hamartin/TSC1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 29906-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 29906-1-AP (Hamartin/TSC1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 29906-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 29906-1-AP (Hamartin/TSC1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Mausnierengewebe, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: Mausnierengewebe, Maus-Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

29906-1-AP bindet in WB, IHC, ELISA Hamartin/TSC1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Hamartin/TSC1 fusion protein Ag31621
• Vollständiger Name: tuberous sclerosis 1
• Berechnetes Molekulargewicht: 130KD
• Beobachtetes Molekulargewicht: 140-145 kDa
• GenBank-Zugangsnummer: NM_000368
• Gene symbol: TSC1
• Gene ID (NCBI): 7248
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TSC1, also named as hamartin, interacts with TSC2 to form a protein complex that inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling (PubMed: 12271141, PubMed: 28215400). It can recruit TSC2 to HSP90AA1 and stabilizes TSC2 by preventing the interaction between TSC2 and ubiquitin ligase HERC1 (PubMed: 16464865, PubMed: 29127155). The loss of TSC1/TSC2 in mouse neurons resulted in a block in oligodendrocyte hypomyelination in vivo (PubMed: 28183733).

Publikationen

Species	Application	Title
human	WB	Elife; A cleaved METTL3 potentiates the METTL3-WTAP interaction and breast cancer progression; Authors - Chaojun Yan