Tuberin/TSC2 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

Tuberin/TSC2 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1A10B2

Kat-Nr. : 68380-1-PBS

Synonyme

TSC2, Tuberin, 1A10B2, TSC4, tuberous sclerosis 2

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  WB analysis using 68380-1-Ig (same clone as 68380-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68380-1-Ig (TSC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68380-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 68380-1-Ig (same clone as 68380-1-PBS)

  WB result of Tuberin/TSC2 antibody (68380-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-Tuberin/TSC2 transfected HeLa cells. This data was developed using the same antibody clone with 68380-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 68380-1-Ig (same clone as 68380-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 68380-1-Ig (TSC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68380-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-3 using 68380-1-Ig (same clone as 68380-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 68380-1-Ig (TSC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68380-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 68380-1-Ig (same clone as 68380-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68380-1-Ig (TSC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68380-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 68380-1-Ig (same clone as 68380-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 68380-1-Ig (TSC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68380-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 68380-1-Ig (same clone as 68380-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68380-1-Ig (Tuberin/TSC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68380-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 68380-1-Ig (same clone as 68380-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68380-1-Ig (Tuberin/TSC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68380-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 68380-1-Ig (same clone as 68380-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68380-1-Ig (Tuberin/TSC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68380-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 68380-1-Ig (same clone as 68380-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68380-1-Ig (Tuberin/TSC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68380-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 68380-1-Ig (same clone as 68380-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using TSC2 antibody (68380-1-Ig, Clone: 1A10B2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68380-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68380-1-PBS bindet in WB, IHC, IF/ICC, ELISA Tuberin/TSC2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: tuberous sclerosis 2
• Berechnetes Molekulargewicht: 1807 aa, 201 kDa
• Beobachtetes Molekulargewicht: 200 kDa
• GenBank-Zugangsnummer: BC150300
• Gene symbol: TSC2
• Gene ID (NCBI): 7249
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TSC2, also named Tuberin, plays an essential role in the cellular energy response pathway. TSC2 forms a physical and functional complex with TSC1 that can inhibit the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling.