TSC22D1 Polyklonaler Antikörper

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• KD/KO Validated

TSC22D1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10214-1-PBS

Synonyme

RP11 269C23.2, Regulatory protein TSC-22, Regulatory protein TSC 22, KIAA1994, HUCEP-2

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  WB analysis of human brain using 10214-1-Ig (same clone as 10214-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 10214-1-Ig (TSC22D1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10214-1-PBS in a different storage buffer formulation.

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  WB analysis of rat brain using 10214-1-Ig (same clone as 10214-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 10214-1-Ig (TSC22D1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10214-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 10214-1-Ig (same clone as 10214-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 10214-1-Ig (TSC22D1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10214-1-PBS in a different storage buffer formulation.

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  IP experiment of rat brain using 10214-1-Ig (same clone as 10214-1-PBS)

  IP result of anti-TSC22D1 (IP:10214-1-Ig, 4ug; Detection:10214-1-Ig 1:500) with rat brain tissue lysate 4000ug. This data was developed using the same antibody clone with 10214-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 10214-1-Ig (same clone as 10214-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10214-1-Ig (TSC22D1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10214-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 10214-1-Ig (same clone as 10214-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10214-1-Ig (TSC22D1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10214-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 10214-1-Ig (same clone as 10214-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10214-1-Ig (TSC22D1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10214-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 10214-1-Ig (same clone as 10214-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10214-1-Ig (TSC22D1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10214-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 10214-1-Ig (same clone as 10214-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10214-1-Ig (TSC22D1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10214-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 10214-1-Ig (same clone as 10214-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10214-1-Ig (TSC22D1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10214-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 10214-1-Ig (same clone as 10214-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using TSC22D1 antibody (10214-1-Ig) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 10214-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10214-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA TSC22D1 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TSC22D1 fusion protein Ag0287
• Vollständiger Name: TSC22 domain family, member 1
• Berechnetes Molekulargewicht: 16 kDa
• Beobachtetes Molekulargewicht: 16 kDa
• GenBank-Zugangsnummer: BC000456
• Gene symbol: TSC22D1
• Gene ID (NCBI): 8848
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TSC22 domain family 1, transforming growth factor beta 1 induced transcript 4, (TSC22D1, synonyms: TSC22, TGFB1I4, MGC17597) is a member of a family of leucine zipper containing transcription factors with repressor activity and belongs to the large family of early response genes. TSC-22 regulates cell growth and differentiation, and cell death. Leucine zipper structure of TSC22 inhibits the anchorage independent growth of cancer cells. TSC-22 is also an important downstream component of peroxisome proliferator-activated receptor (PPAR) and TGF-beta signaling during intestinal epithelial cell differentiation. This antibody is a rabbit polyclonal antibody raised against an internal region of human TSC22D1.

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