TSC22D2 Polyklonaler Antikörper

TSC22D2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 25418-1-PBS

Synonyme

TSC22 domain family protein 2, TILZ4c, TILZ4b, TILZ4a, TILZ4

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  WB analysis using 25418-1-AP (same clone as 25418-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 25418-1-AP (TSC22D2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25418-1-PBS in a different storage buffer formulation.

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  WB analysis of MDA-MB-453s using 25418-1-AP (same clone as 25418-1-PBS)

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 25418-1-AP (TSC22D2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25418-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 25418-1-AP (same clone as 25418-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25418-1-AP (TSC22D2 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 25418-1-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 25418-1-AP (same clone as 25418-1-PBS)

  IP result of anti-TSC22D2 (IP:25418-1-AP, 5ug; Detection:25418-1-AP 1:300) with HepG2 cells lysate 2500ug. This data was developed using the same antibody clone with 25418-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 25418-1-AP (same clone as 25418-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25418-1-AP (TSC22D2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 25418-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 25418-1-AP (same clone as 25418-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25418-1-AP (TSC22D2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 25418-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 25418-1-AP (same clone as 25418-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25418-1-AP (TSC22D2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 25418-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 25418-1-AP (same clone as 25418-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25418-1-AP (TSC22D2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 25418-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 25418-1-AP (same clone as 25418-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TSC22D2 antibody (25418-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 25418-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

25418-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA TSC22D2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TSC22D2 fusion protein Ag22294
• Vollständiger Name: TSC22 domain family, member 2
• Berechnetes Molekulargewicht: 780 aa, 79 kDa
• Beobachtetes Molekulargewicht: 100-110 kDa
• GenBank-Zugangsnummer: BC044643
• Gene symbol: TSC22D2
• Gene ID (NCBI): 9819
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TSC22D2 (transforming growth factor β-stimulated clone 22 domain family, member 2) is a member of the TSC-22 domain family comprising putative transcription factors that are characterized by a carboxy-terminal leucine zipper and an adjacent TSC-box. TSC22D2 is expressed at low levels in CRC and that TSC22D2 overexpression significantly inhibited the growth rate of cancer cells (PMID: 27573352).