GILZ Polyklonaler Antikörper

GILZ Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 12352-1-AP

Synonyme

TSC22D3, TSC22 D3, TSC 22R, DSIP-immunoreactive peptide, DSIPI

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  WB analysis of mouse brain using 12352-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12352-1-AP (GILZ antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12352-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12352-1-AP (GILZ antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12352-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12352-1-AP (GILZ antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse liver using 12352-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 12352-1-AP (GILZ antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of U2OS using 12352-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using GILZ antibody (12352-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe
• Erfolgreiche Detektion in IHC: Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below

Produktinformation

12352-1-AP bindet in WB, IHC, IF/ICC, ELISA GILZ und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GILZ fusion protein Ag3015
• Vollständiger Name: TSC22 domain family, member 3
• Berechnetes Molekulargewicht: 134 aa, 15 kDa
• Beobachtetes Molekulargewicht: 17 kDa
• GenBank-Zugangsnummer: BC018148
• Gene symbol: GILZ
• Gene ID (NCBI): 1831
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GILZ, also known as TSC22D3 and DSIPI, is a glucocorticoid responsive protein and is thought to mediate some of the anti-inflammatory effects of glucocorticoid receptors (GRs). GILZ is mainly localized in the cytoplasm. It is an anti-inflammatory molecule that could mediate the immunomodulatory activities of GCs and also plays a role in cell differentiation in various cells and tissues (PMID: 35011684).

Publikationen

Species	Application	Title
human	WB	J Adv Res; Mini and enhanced CRISPR activators for cancer therapies; Authors - Meiyu Huang
rat	WB	Invest Ophthalmol Vis Sci; Glucocorticoid-Induced Leucine Zipper Protects the Retina From Light-Induced Retinal Degeneration by Inducing Bcl-xL in Rats.; Authors - Ruiping Gu
rat	WB	Invest Ophthalmol Vis Sci; Glucocorticoid-Induced Leucine Zipper Suppresses ICAM-1 and MCP-1 Expression by Dephosphorylation of NF-κB p65 in Retinal Endothelial Cells.; Authors - Ruiping Gu
human	WB	Exp Eye Res; Glucocorticoid-induced leucine zipper overexpression inhibits lipopolysaccharide-induced retinal inflammation in rats.; Authors - Ruiping Gu