GILZ Polyklonaler Antikörper

GILZ Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 12352-1-AP

Synonyme

DIP, DSIP immunoreactive peptide, DSIPI, GILZ, hDIP, Protein DIP, TSC 22 like protein, TSC 22 related protein, TSC 22R, TSC22 domain family protein 3, TSC22 domain family, member 3, TSC22D3

---

Galerie der Validierungsdaten

• 

  WB analysis of mouse brain using 12352-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12352-1-AP (GILZ antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12352-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12352-1-AP (GILZ antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12352-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12352-1-AP (GILZ antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IF Staining of U2OS using 12352-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using GILZ antibody (12352-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe
• Erfolgreiche Detektion in IF: U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

12352-1-AP bindet in WB, IF, ELISA GILZ und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GILZ fusion protein Ag3015
• Vollständiger Name: TSC22 domain family, member 3
• Berechnetes Molekulargewicht: 134 aa, 15 kDa
• Beobachtetes Molekulargewicht: 17 kDa
• GenBank-Zugangsnummer: BC018148
• Gene symbol: TSC22D3
• Gene ID (NCBI): 1831
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
rat	WB	Invest Ophthalmol Vis Sci; Glucocorticoid-Induced Leucine Zipper Protects the Retina From Light-Induced Retinal Degeneration by Inducing Bcl-xL in Rats.; Authors - Ruiping Gu
rat	WB	Invest Ophthalmol Vis Sci; Glucocorticoid-Induced Leucine Zipper Suppresses ICAM-1 and MCP-1 Expression by Dephosphorylation of NF-κB p65 in Retinal Endothelial Cells.; Authors - Ruiping Gu
human	WB	Exp Eye Res; Glucocorticoid-induced leucine zipper overexpression inhibits lipopolysaccharide-induced retinal inflammation in rats.; Authors - Ruiping Gu