TSEN34 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TSEN34 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 31212-1-AP

Synonyme

EC:4.6.1.16, HsSen34, LENG5, PCH2C, SEN34

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  WB analysis of MCF-7 using 31212-1-AP

  WB result of TSEN34 antibody (31212-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TSEN34 transfected MCF-7 cells.

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  WB analysis using 31212-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31212-1-AP (TSEN34 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 31212-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31212-1-AP (TSEN34 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 31212-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using TSEN34 antibody (31212-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HCT 116-Zellen, HEK-293-Zellen, HEK-293T-Zellen, HeLa-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

31212-1-AP bindet in WB, IF/ICC, ELISA TSEN34 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TSEN34 fusion protein Ag35120
• Vollständiger Name: tRNA splicing endonuclease 34 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 34 kDa
• Beobachtetes Molekulargewicht: 34-36 kDa
• GenBank-Zugangsnummer: BC020805
• Gene symbol: TSEN34
• Gene ID (NCBI): 79042
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The human tRNA splicing endonuclease consists of a heterotetramer complex formed by the four TSEN proteins, which were identified by homology with their yeast counterparts. TSEN2 and TSEN34 are the catalytic subunits, which form a compound active site, whereas TSEN54 and TSEN15 are structural proteins. (PMID: 27392077)