THEMIS Polyklonaler Antikörper

THEMIS Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 27415-1-AP

Synonyme

TSEPA, Thymocyte-expressed molecule involved in selection, Protein THEMIS, C6orf207, C6orf190

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  WB analysis of Jurkat using 27415-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 27415-1-AP (TSEPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 27415-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27415-1-AP (TSEPA antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 27415-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27415-1-AP (TSEPA antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 27415-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 27415-1-AP (TSEPA antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 27415-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 27415-1-AP (TSEPA antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, Mausmilzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

27415-1-AP bindet in WB, IHC, IF, ELISA THEMIS und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: THEMIS fusion protein Ag26387
• Vollständiger Name: thymocyte selection pathway associated
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC130516
• Gene symbol: THEMIS
• Gene ID (NCBI): 387357
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The T cell lineage-restricted protein THEMIS, also called C6orf190 and C6orf207, plays a critical role in T cell development at the positive selection stage (PMID: 37159521). THEMIS plays a central role in late thymocyte development by controlling both positive and negative T-cell selection. It is required to sustain and/or integrate signals required for proper lineage commitment and maturation of T-cells and regulates T-cell development through T-cell antigen receptor (TCR) signaling (PMID: 28697966, 28250424). It can also promote the proliferative response of CD8+ T cells to low-affinity ligands (PMID: 31932808).

Publikationen

Species	Application	Title
human,mouse	IF	Theranostics; Single cell analyses reveal the PD-1 blockade response-related immune features in hepatocellular carcinoma; Authors - Yao Li