TSFM Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TSFM Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 11701-1-AP

Synonyme

EF-TsMt, EF-Ts, EF Tsmt, EF TS, COXPD3

---

• 

  WB analysis of HeLa using 11701-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11701-1-AP (TSFM Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11701-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11701-1-AP (TSFM Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 11701-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11701-1-AP (TSFM Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 11701-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11701-1-AP (TSFM antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon cancer using 11701-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11701-1-AP (TSFM antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human stomach cancer using 11701-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11701-1-AP (TSFM antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human stomach cancer using 11701-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11701-1-AP (TSFM antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human kidney using 11701-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11701-1-AP (TSFM antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 11701-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11701-1-AP (TSFM antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HeLa using 11701-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 11701-1-AP (TSFM antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Nierengewebe, humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below

Produktinformation

11701-1-AP bindet in WB, IHC, IF/ICC, ELISA TSFM und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TSFM fusion protein Ag2308
• Vollständiger Name: Ts translation elongation factor, mitochondrial
• Berechnetes Molekulargewicht: 35 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC022862
• Gene symbol: TSFM
• Gene ID (NCBI): 10102
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Mol Biol Rep; Short-term regulation of TSFM level does not alter amyloidogenesis and mitochondrial function in type-specific cells; Authors - Xiao-Yun Li ; KD Validated
human	WB	Cell Death Dis; piR-26441 inhibits mitochondrial oxidative phosphorylation and tumorigenesis in ovarian cancer through m6A modification by interacting with YTHDC1; Authors - Jing Yuan