TSG-6 Rekombinanter Antikörper

TSG-6 Rekombinant Antikörper für WB, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

230438D5

Kat-Nr. : 83163-4-RR

Synonyme

TNFAIP6, TNF alpha-induced protein 6, TNF alpha induced protein 6, Hyaluronate-binding protein, Hyaluronate binding protein

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  WB analysis using 83163-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83163-4-RR (TNFAIP6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of A549 using 83163-4-RR

  1x10^6 A549 cells were intracellularly stained with 0.25 ug TSG-6 Recombinant antibody (83163-4-RR, Clone:230438D5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83163-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83163-4-RR against Human TSG-6 were performed. The affinity constant is 1.93 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HEK-293-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in FC (Intra): A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83163-4-RR bindet in WB, FC (Intra), ELISA TSG-6 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TSG-6 fusion protein Ag3885
• Vollständiger Name: tumor necrosis factor, alpha-induced protein 6
• Berechnetes Molekulargewicht: 277 aa, 31 kDa
• Beobachtetes Molekulargewicht: 39-45 kDa
• GenBank-Zugangsnummer: BC030205
• Gene symbol: TNFAIP6
• Gene ID (NCBI): 7130
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.