TSG101 Polyklonaler Antikörper

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• KD/KO Validated

TSG101 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (6)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(340)

Rezensionen (2)

Kat-Nr. : 28283-1-AP

Synonyme

ESCRT-I complex subunit TSG101, TSG 10, TSG 101, TSG10, VPS 23

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  WB analysis using 28283-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28283-1-AP (TSG101 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 28283-1-AP

  IP result of anti-TSG101 (IP:28283-1-AP, 4ug; Detection:28283-1-AP 1:2000) with HeLa cells lysate 1280 ug.

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  IHC staining of human lung cancer using 28283-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28283-1-AP (TSG101 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 28283-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28283-1-AP (TSG101 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 28283-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28283-1-AP (TSG101 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 28283-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28283-1-AP (TSG101 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 28283-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TSG101 antibody (28283-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, NIH/3T3-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Kolonkarzinomgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 330 publications below
• IHC: See 2 publications below
• IF: See 5 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below

Produktinformation

28283-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA TSG101 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Affe, hamster, Huhn, Kaninchen, Maus, Ratte, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TSG101 fusion protein Ag28569
• Vollständiger Name: tumor susceptibility gene 101
• Berechnetes Molekulargewicht: 44 kDa
• Beobachtetes Molekulargewicht: 44 kDa
• GenBank-Zugangsnummer: BC002487
• Gene symbol: TSG101
• Gene ID (NCBI): 7251
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TSG101(Tumor susceptibility gene 101 protein) is essential for endosomal sorting, membrane receptor degradation and the final stages of cytokinesis. It plays a crucial role for cell proliferation and cell survival. TSG101 has been identified as a candidate tumor suppressor gene and belongs to the ubiquitin-conjugating enzyme family. TSG101 is a marker for exosome. This protein has 2 isoforms produced by alternative splicing with the molecular mass of 44 and 32 kDa.

Publikationen

Species	Application	Title
human	WB	Cell Res; In vivo self-assembled small RNAs as a new generation of RNAi therapeutics.; Authors - Zheng Fu
rat	WB	Nat Commun; Injectable ECM-mimetic dynamic hydrogels abolish ferroptosis-induced post-discectomy herniation through delivering nucleus pulposus progenitor cell-derived exosomes; Authors - Wenkai Wang
human	WB	J Extracell Vesicles; Quantification of urinary podocyte-derived migrasomes for the diagnosis of kidney disease; Authors - Rong Yang
human	WB	Adv Sci (Weinh); LIMA1 O-GlcNAcylation Promotes Hepatic Lipid Deposition through Inducing β-catenin-Regulated FASn Expression in Metabolic Dysfunction-Associated Steatotic Liver Disease; Authors - Fuji Yang
human	WB	J Am Chem Soc; Efficient Metabolomics Profiling from Plasma Extracellular Vesicles Enables Accurate Diagnosis of Early Gastric Cancer; Authors - Fanqin Bu
human	WB	J Extracell Vesicles; A multi-omics approach identifies pancreatic cancer cell extracellular vesicles as mediators of the unfolded protein response in normal pancreatic epithelial cells.; Authors - Charles P Hinzman

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kamal (Verified Customer) (02-15-2024)

Mouse liver lysates were subjected to SDS PAGE followed by western blot with 28283-1-AP (TSG101 antibody) at dilution of 1:10000 incubated at 4 degree C for 1.5 hours. TSG101 bands appeared at 42 kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:10000
• Cell Tissue Type: Mouse liver tissue

FH

SONAM (Verified Customer) (01-09-2024)

Antibody works well. Ordered three times..

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: cancer cellls, cancer cell derived Exosome