TSG101 Monoklonaler Antikörper

TSG101 Monoklonal Antikörper für WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(59)

CloneNo.

2B7G8

Kat-Nr. : 67381-1-Ig

Synonyme

2B7G8, ESCRT-I complex subunit TSG101, TSG 10, TSG 101, TSG10

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  WB analysis using 67381-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67381-1-Ig (TSG101 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis using 67381-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67381-1-Ig (TSG101 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of human urine exosomes using 67381-1-Ig

  human urine exosomes tissue were subjected to SDS PAGE followed by western blot with 67381-1-Ig (TSG101 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 67381-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67381-1-Ig (TSG101 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 67381-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67381-1-Ig (TSG101 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 67381-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67381-1-Ig (TSG101 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 67381-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67381-1-Ig (TSG101 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human colon cancer using 67381-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using TSG101 antibody (67381-1-Ig, Clone: 2B7G8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of HeLa using 67381-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 67381-1-Ig (TSG101 antibody), at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of HeLa using 67381-1-Ig

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug TSG101 Monoclonal antibody (67381-1-Ig, Clone:2B7G8) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, C6-Zellen, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Kolonkarzinomgewebe
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, humanes Kolonkarzinomgewebe
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:1000-1:4000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 53 publications below
• IF: See 6 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

67381-1-Ig bindet in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA TSG101 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TSG101 fusion protein Ag28569
• Vollständiger Name: tumor susceptibility gene 101
• Berechnetes Molekulargewicht: 44 kDa
• Beobachtetes Molekulargewicht: 43-46 kDa
• GenBank-Zugangsnummer: BC002487
• Gene symbol: TSG101
• Gene ID (NCBI): 7251
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TSG101(Tumor susceptibility gene 101 protein) is essential for endosomal sorting, membrane receptor degradation and the final stages of cytokinesis. It plays a crucial role for cell proliferation and cell survival. TSG101 has been identified as a candidate tumor suppressor gene and belongs to the ubiquitin-conjugating enzyme family. TSG101 is a marker for exosome. This protein has 2 isoforms produced by alternative splicing with the molecular mass of 44 and 32 kDa.

Publikationen

Species	Application	Title
human	WB	Mol Cancer; Exosomal circ_0006896 promotes AML progression via interaction with HDAC1 and restriction of antitumor immunity; Authors - Can Can
mouse,human	WB	J Exp Clin Cancer Res; Chemotherapy-elicited extracellular vesicle CXCL1 from dying cells promotes triple-negative breast cancer metastasis by activating TAM/PD-L1 signaling; Authors - Shengqi Wang
rat	WB	Bioact Mater; Injectable cartilage matrix hydrogel loaded with cartilage endplate stem cells engineered to release exosomes for non-invasive treatment of intervertebral disc degeneration.; Authors - Liwen Luo
human	WB	Arterioscler Thromb Vasc Biol; Exosomes From IgE-Stimulated Mast Cells Aggravate Asthma-Mediated Atherosclerosis Through circRNA CDR1as-Mediated Endothelial Cell Dysfunction in Mice; Authors - Hongqin Yang
human	WB	Stem Cell Res Ther; Tremella polysaccharide microneedles loaded with magnetic dental pulp stem cell intracellular vesicles used for androgenic alopecia; Authors - Yonghao Liu
mouse	WB	Oxid Med Cell Longev; Adipose-Derived Circulating Exosomes Promote Protection of the Pulmonary Endothelial Barrier by Inhibiting EndMT and Oxidative Stress through Down-Regulation of the TGF-β Pathway: A Potential Explanation for the Obesity Paradox in ARDS.; Authors - Di Qi