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TSG101 Rekombinanter Antikörper

TSG101 Rekombinant Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241657A1

Kat-Nr. : 84334-4-PBS

Synonyme

241657A1, ESCRT-I complex subunit TSG101, TSG 10, TSG 101, TSG10

Filter:
  • Western Blot (WB) analysis of various lysates using TSG101 Recombinant antibody (84334-4-RR)

    WB analysis using 84334-4-RR (same clone as 84334-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84334-4-RR (TSG101 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84334-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TSG101 Recombinant antibody (84334-4-RR)

    IHC staining of human colon cancer using 84334-4-RR (same clone as 84334-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84334-4-RR (TSG101 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84334-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TSG101 Recombinant antibody (84334-4-RR)

    IF Staining of HeLa using 84334-4-RR (same clone as 84334-4-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TSG101 antibody (84334-4-RR, Clone: 241657A1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84334-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84334-4-RR

    Affinity and Kinetic Characterization of 84334-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84334-4-RR against Human TSG101 were performed. The affinity constant is below 1 pM.

Geprüfte Anwendungen

Produktinformation

84334-4-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA TSG101 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen TSG101 fusion protein Ag28569
Vollständiger Name tumor susceptibility gene 101
Berechnetes Molekulargewicht 44 kDa
Beobachtetes Molekulargewicht44 kDa
GenBank-ZugangsnummerBC002487
Gene symbol TSG101
Gene ID (NCBI) 7251
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TSG101(Tumor susceptibility gene 101 protein) is essential for endosomal sorting, membrane receptor degradation and the final stages of cytokinesis. It plays a crucial role for cell proliferation and cell survival. TSG101 has been identified as a candidate tumor suppressor gene and belongs to the ubiquitin-conjugating enzyme family. TSG101 is a marker for exosome. This protein has 2 isoforms produced by alternative splicing with the molecular mass of 44 and 32 kDa.