TSGA10 Polyklonaler Antikörper

TSGA10 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Kat-Nr. : 12593-1-AP

Synonyme

CEP4L, testis specific, 10, TSGA10

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  WB analysis of rat testis using 12593-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 12593-1-AP (TSGA10 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 12593-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12593-1-AP (TSGA10 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 12593-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 12593-1-AP (TSGA10 Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 12593-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12593-1-AP (TSGA10 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human testis using 12593-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12593-1-AP (TSGA10 Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenhodengewebe, Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Produktinformation

12593-1-AP bindet in WB, IF, IHC, ELISA TSGA10 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TSGA10 fusion protein Ag3311
• Vollständiger Name: testis specific, 10
• Berechnetes Molekulargewicht: 698 aa, 81 kDa
• Beobachtetes Molekulargewicht: 81 kDa
• GenBank-Zugangsnummer: BC028366
• Gene symbol: TSGA10
• Gene ID (NCBI): 80705
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB,IHC	Front Cell Dev Biol; miR-129-5p Inhibits Bone Formation Through TCF4.; Authors - Chong Yin
human	WB	Front Cell Dev Biol; Pathogenic Variants in ACTRT1 Cause Acephalic Spermatozoa Syndrome.; Authors - Yanwei Sha
human,mouse	WB，IHC	J Cell Mol Med; MACF1 alleviates aging-related osteoporosis via HES1.; Authors - Chong Yin
human	WB,IF	Clin Genet; TSGA10 is a novel candidate gene associated with acephalic spermatozoa.; Authors - Yan-Wei Sha
human	WB	J Assist Reprod Genet; Pathogenesis of acephalic spermatozoa syndrome caused by splicing mutation and de novo deletion in TSGA10.; Authors - Mingfei Xiang
human	WB,IF	Basic Clin Androl; A novel homozygous TSGA10 missense variant causes acephalic spermatozoa syndrome in a Pakistani family; Authors - Khalid Khan