TSHR Polyklonaler Antikörper

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TSHR Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(20)

Kat-Nr. : 14450-1-AP

Synonyme

TSH R, Thyrotropin receptor, Thyroid-stimulating hormone receptor, LGR3, hTSHR I

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  WB analysis of HeLa using 14450-1-AP

  WB result of TSHR antibody (14450-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-TSHR transfected HeLa cells.

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  WB analysis using 14450-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14450-1-AP (TSHR antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 14450-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14450-1-AP (TSHR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 14450-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 14450-1-AP (TSHR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human thyroid cancer using 14450-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 14450-1-AP (TSHR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: TT cells, HeLa-Zellen, Maus-Eierstockgewebe, Maus-Thymusgewebe
• Erfolgreiche Detektion in IHC: humanes Schilddrüsenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 12 publications below
• IHC: See 2 publications below
• IF: See 6 publications below

Produktinformation

14450-1-AP bindet in WB, IHC, IF, ELISA TSHR und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TSHR fusion protein Ag5957
• Vollständiger Name: thyroid stimulating hormone receptor
• Berechnetes Molekulargewicht: 87 kDa
• Beobachtetes Molekulargewicht: 87kDa, 62-70kDa, 28-30kDa
• GenBank-Zugangsnummer: BC063613
• Gene symbol: TSHR
• Gene ID (NCBI): 7253
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Thyroid stimulating hormone (also known as thyrotropin or TSH) is a glycoprotein hormone produced by thyrotrophs in the anterior pituitary gland. The TSH receptor (TSHR), mainly expressed on the thyroid epithelial cells, is a G protein-coupled receptor (GPCR) of the seven-transmembrane domain family and plays a central role in controlling thyroid cell metabolism. The gene of TSHR maps to chromosome 14q31.1, and encodes a 764-amino acid protein with a predicted unmodified molecular weight of 86.8 kDa. The apparent molecular weight of glycosylated form is 95-120 kDa. Studies reveal that TSHR is composed of two subunits, A and B, which are produced by cleavage of single-chain TSHR on the cell surface and subsequently connected by disulfide bonds. This rabbit polyclonal antibody raised against N-terminal region (1-253 aa) of human TSHR can detect glycosylated subunit A with an experimentally determined molecular weight of 62-70 kDa under reducing condition. (PMID: 20578897, 17158770, 12593718)

Publikationen

Species	Application	Title
human	WB	Theranostics; Obatoclax and LY3009120 Efficiently Overcome Vemurafenib Resistance in Differentiated Thyroid Cancer.; Authors - Wei-Jun Wei
human	IF	Int J Nanomedicine; A TSHR-Targeting Aptamer in Monocytes Correlating with Clinical Activity in TAO; Authors - Jiamin Cao
mouse	IHC	Oxid Med Cell Longev; TSH Combined with TSHR Aggravates Diabetic Peripheral Neuropathy by Promoting Oxidative Stress and Apoptosis in Schwann Cells.; Authors - Jingwen Fan
zebrafish	WB	Environ Pollut; Acute exposure of zebrafish embryo (Danio rerio) to flutolanil reveals its developmental mechanism of toxicity via disrupting the thyroid system and metabolism.; Authors - Miaomiao Teng
human	IF	Cancers (Basel); Patient-Derived Papillary Thyroid Cancer Organoids for Radioactive Iodine Refractory Screening.; Authors - Luc H J Sondorp
human	WB	Front Endocrinol (Lausanne); Long Non-coding Antisense RNA TNRC6C-AS1 Is Activated in Papillary Thyroid Cancer and Promotes Cancer Progression by Suppressing TNRC6C Expression.; Authors - Dilidaer Muhanhali