TSPAN1 Polyklonaler Antikörper

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• KD/KO Validated

TSPAN1 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16058-1-PBS

Synonyme

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  WB analysis using 16058-1-AP (same clone as 16058-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16058-1-AP (TSPAN1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16058-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 16058-1-AP (same clone as 16058-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 16058-1-AP (TSPAN1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16058-1-PBS in a different storage buffer formulation.

• 

  WB analysis of COLO 320 using 16058-1-AP (same clone as 16058-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 16058-1-AP (TSPAN1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16058-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat colon using 16058-1-AP (same clone as 16058-1-PBS)

  rat colon tissue were subjected to SDS PAGE followed by western blot with 16058-1-AP (TSPAN1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16058-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 16058-1-AP (same clone as 16058-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16058-1-AP (TSPAN1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16058-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 16058-1-AP (same clone as 16058-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16058-1-AP (TSPAN1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16058-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

16058-1-PBS bindet in WB, IHC, Indirect ELISA TSPAN1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TSPAN1 fusion protein Ag8786
• Vollständiger Name: tetraspanin 1
• Berechnetes Molekulargewicht: 241 aa, 26 kDa
• Beobachtetes Molekulargewicht: 26-30 kDa
• GenBank-Zugangsnummer: BC013404
• Gene symbol: TSPAN1
• Gene ID (NCBI): 10103
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TSPAN1 is a membrane protein of the tetraspanin superfamily, which is characterized by the presence of four conserved transmembrane regions. Tetraspanins have been involved in diverse processes such as cell activation and proliferation, adhesion and motility, differentiation, and cancer. Altered TSPAN1 expression has been associated with the progression of some neoplasms including hepatocellular carcinoma, colorectal, gastric, and breast cancers (PMID: 20890423; 19437569; 18822690; 20680643).