TSPAN31 Polyklonaler Antikörper

TSPAN31 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 21987-1-AP

Synonyme

Sarcoma amplified sequence, SAS, tetraspanin 31, Tspan 31, TSPAN31

---

• 

  WB analysis of HeLa using 21987-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21987-1-AP (TSPAN31 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 21987-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21987-1-AP (TSPAN31 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 21987-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 21987-1-AP (TSPAN31 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 21987-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21987-1-AP (TSPAN31 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skeletal muscle using 21987-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 21987-1-AP (TSPAN31 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 21987-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 21987-1-AP (TSPAN31 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human heart using 21987-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 21987-1-AP (TSPAN31 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human heart using 21987-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 21987-1-AP (TSPAN31 Antibody) at dilution of 1:200 (under 40x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, Maus-Skelettmuskelgewebe, PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Skelettmuskelgewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 2 publications below

Produktinformation

21987-1-AP bindet in WB, IHC, ELISA TSPAN31 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TSPAN31 fusion protein Ag17138
• Vollständiger Name: tetraspanin 31
• Berechnetes Molekulargewicht: 210 aa, 23 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC010377
• Gene symbol: TSPAN31
• Gene ID (NCBI): 6302
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tetraspanin-31 (TSPAN31) belongs to the tetraspanin (TM4SF) family, also known as the transmembrane 4 (TM4) superfamily, which are a family of transmembrane proteins with 33 mammalian members. These proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. Tetraspanin-31 is thought to be involved in growth-related cellular processes. The experimentally determined molecular mass of 30 kDa is larger than the calculated molecular mass of 23 kDa, which could be a result of post-translational modifications.

Publikationen

Species	Application	Title
human	IHC	Cancer Sci; Overexpression of Tetraspanin31 contributes to malignant potential and poor outcomes in gastric cancer; Authors - Yusuke Takashima
human	WB,IHC	Mol Carcinog; TSPAN31 Activates Fatty Acid Metabolism and PI3K/AKT Pathway to Promote Tumor Progression in Breast Cancer; Authors - Wenquan Luo