TSR2 Polyklonaler Antikörper

TSR2 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 16263-1-AP

Synonyme

DT1P1A10, TSR2, WGG1

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Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 16263-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16263-1-AP (TSR2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 16263-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16263-1-AP (TSR2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 16263-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16263-1-AP (TSR2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 16263-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 16263-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 16263-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 16263-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 16263-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 16263-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human epididymis using 16263-1-AP

  Immunohistochemical analysis of paraffin-embedded human epididymis using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human epididymis using 16263-1-AP

  Immunohistochemical analysis of paraffin-embedded human epididymis using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 16263-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 16263-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 16263-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 16263-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 16263-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 16263-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of MCF-7 using 16263-1-AP

  Immunofluorescent analysis of MCF-7 cells, using TSR2 antibody 16263-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Herzgewebe, humanes Lungengewebe, humanes Milzgewebe, humanes Nebenhodengewebe, humanes Nierengewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 2 publications below

Produktinformation

16263-1-AP bindet in WB, IHC, IF, ELISA TSR2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TSR2 fusion protein Ag9328
• Vollständiger Name: TSR2, 20S rRNA accumulation, homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 191 aa, 21 kDa
• Beobachtetes Molekulargewicht: 24-30 kDa
• GenBank-Zugangsnummer: BC011825
• Gene symbol: TSR2
• Gene ID (NCBI): 90121
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	IHC	Acta Histochem; Ultrastructural analysis of human leukemia U-937 cells after apoptosis induction: Localization of proteasomes and perichromatin fibers.; Authors - Ekaterina S Snigirevskaya
human	IHC	Cell Biol Int; Ultrastructural traits of apoptosis; Authors - Ekaterina S Snigirevskaya