TSR2 Polyklonaler Antikörper

TSR2 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 16263-1-AP

Synonyme

WGG1, Pre-rRNA-processing protein TSR2 homolog, DT1P1A10

---

• 

  WB analysis of HeLa using 16263-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16263-1-AP (TSR2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 16263-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16263-1-AP (TSR2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 16263-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16263-1-AP (TSR2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 16263-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 16263-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 16263-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 16263-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 16263-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 16263-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 16263-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 16263-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 16263-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 16263-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 16263-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 16263-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human epididymis using 16263-1-AP

  Immunohistochemical analysis of paraffin-embedded human epididymis using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human epididymis using 16263-1-AP

  Immunohistochemical analysis of paraffin-embedded human epididymis using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of MCF-7 using 16263-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using TSR2 antibody (16263-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Nebenhodengewebe, humanes Herzgewebe, humanes Nierengewebe, humanes Lungengewebe, humanes Plazenta-Gewebe, humanes Milzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 2 publications below

Produktinformation

16263-1-AP bindet in WB, IHC, IF/ICC, ELISA TSR2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TSR2 fusion protein Ag9328
• Vollständiger Name: TSR2, 20S rRNA accumulation, homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 191 aa, 21 kDa
• Beobachtetes Molekulargewicht: 24-30 kDa
• GenBank-Zugangsnummer: BC011825
• Gene symbol: TSR2
• Gene ID (NCBI): 90121
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	IHC	Acta Histochem; Ultrastructural analysis of human leukemia U-937 cells after apoptosis induction: Localization of proteasomes and perichromatin fibers.; Authors - Ekaterina S Snigirevskaya
human	IHC	Cell Biol Int; Ultrastructural traits of apoptosis; Authors - Ekaterina S Snigirevskaya