TST Polyklonaler Antikörper

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• KD/KO Validated

TST Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(9)

Kat-Nr. : 16311-1-AP

Synonyme

Rhodanese, RDS, EC:2.8.1.1

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  WB analysis of liver tissue using 16311-1-AP

  WB result of anti-TST (16311-1-AP) in Liver by Dr. Seow Chong Lee.

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  WB analysis using 16311-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16311-1-AP (TST antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 16311-1-AP

  WB result of TST antibody (16311-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TST transfected HEK-293 cells.

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  WB analysis of human kidney using 16311-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 16311-1-AP (TST antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 16311-1-AP

  IP result of anti-TST (IP:16311-1-AP, 4ug; Detection:16311-1-AP 1:300) with HEK-293 cells lysate 2800ug.

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  IHC staining of human colon cancer using 16311-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 16311-1-AP (TST antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 16311-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 16311-1-AP (TST antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse kidney using 16311-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16311-1-AP (TST antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 16311-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16311-1-AP (TST antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, humanes Nierengewebe
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 9 publications below

Produktinformation

16311-1-AP bindet in WB, IHC, IP, ELISA TST und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TST fusion protein Ag9346
• Vollständiger Name: thiosulfate sulfurtransferase (rhodanese)
• Berechnetes Molekulargewicht: 297 aa, 33 kDa
• Beobachtetes Molekulargewicht: 33 kDa
• GenBank-Zugangsnummer: BC010148
• Gene symbol: TST
• Gene ID (NCBI): 7263
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Thiosulfate sulfurtransferase (TST), also known as rhodanese, is a mitochondrial enzyme involved in cyanide detoxification and the formation of iron-sulfur proteins and the modification of sulfur-containing enzymes.

Publikationen

Species	Application	Title
human	WB	EMBO Mol Med; Coenzyme Q deficiency causes impairment of the sulfide oxidation pathway.; Authors - Marcello Ziosi
mouse	WB	EMBO Mol Med; CoQ deficiency causes disruption of mitochondrial sulfide oxidation, a new pathomechanism associated with this syndrome.; Authors - Marta Luna-Sánchez
mouse	WB	Antioxid Redox Signal; The Cysteine Dioxgenase Knockout Mouse: Altered Cysteine Metabolism in Nonhepatic Tissues Leads to Excess H2S/HS(-) Production and Evidence of Pancreatic and Lung Toxicity.; Authors - Roman Heather B HB
mouse,human	WB	Hum Mol Genet; Coenzyme Q10 modulates sulfide metabolism and links the mitochondrial respiratory chain to pathways associated to one carbon metabolism.; Authors - Pilar González-García
mouse	WB	Biochem Pharmacol; Cystathionine-γ-lyase (CSE) deficiency increases erythropoiesis and promotes mitochondrial electron transport via the upregulation of coproporphyrinogen III oxidase and consequent stimulation of heme biosynthesis.; Authors - Katalin Módis
mouse	WB	Amino Acids; Primary hepatocytes from mice lacking cysteine dioxygenase show increased cysteine concentrations and higher rates of metabolism of cysteine to hydrogen sulfide and thiosulfate.; Authors - Halina Jurkowska