TST Monoklonaler Antikörper

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• KD/KO Validated

TST Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

CloneNo.

2A6C5

Kat-Nr. : 66018-1-Ig

Synonyme

2A6C5, EC:2.8.1.1, RDS, Rhodanese

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  WB analysis using 66018-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66018-1-Ig (TST antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66018-1-Ig

  WB result of TST antibody (66018-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TST transfected HEK-293 cells.

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  WB analysis of rat liver using 66018-1-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 66018-1-Ig (TST Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 66018-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 66018-1-Ig (TST antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 66018-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66018-1-Ig (TST Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 66018-1-Ig

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 66018-1-Ig (TST Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat kidney using 66018-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 66018-1-Ig (TST antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66018-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66018-1-Ig (TST antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 66018-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney using 66018-1-Ig(TST antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 66018-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney using 66018-1-Ig(TST antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of A549 using 66018-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using TST antibody (66018-1-Ig, Clone: 2A6C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HEK-293-Zellen, humanes Hirngewebe, Maushirngewebe, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: Rattennierengewebe, humanes Nierengewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:8000-1:32000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below
• IHC: See 1 publications below

Produktinformation

66018-1-Ig bindet in WB, IHC, IF/ICC, ELISA TST und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TST fusion protein Ag9235
• Vollständiger Name: thiosulfate sulfurtransferase (rhodanese)
• Berechnetes Molekulargewicht: 297 aa, 33 kDa
• Beobachtetes Molekulargewicht: 33 kDa
• GenBank-Zugangsnummer: BC010148
• Gene symbol: TST
• Gene ID (NCBI): 7263
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Thiosulfate sulfurtransferase (TST), also known as rhodanese, is a mitochondrial enzyme involved in cyanide detoxification and the formation of iron-sulfur proteins and the modification of sulfur-containing enzymes.

Publikationen

Species	Application	Title
mouse,human	WB	J Biol Chem; Polymorphic variants of human rhodanese exhibit differences in thermal stability and sulfurtransfer kinetics.; Authors - Marouane Libiad
human	WB	Biochem Pharmacol; Drug resistance induces the upregulation of H2S-producing enzymes in HCT116 colon cancer cells.; Authors - Ashley A Untereiner
human	IHC	J Biol Chem; Hydrogen sulfide perturbs mitochondrial bioenergetics and triggers metabolic reprogramming in colon cells.; Authors - Marouane Libiad
human	WB	Biomolecules; Sulfurtransferases and Cystathionine Beta-Synthase Expression in Different Human Leukemia Cell Lines.; Authors - Halina Jurkowska
rat	WB	Mol Biol Rep; Curcumin reduces myocardial ischemia-reperfusion injury, by increasing endogenous H2S levels and further modulating m6A; Authors - Jiankun Cui
mouse	WB	Burns; Cystathionine-gamma-lyase deficient mice are protected against the development of multiorgan failure and exhibit reduced inflammatory response during burn.; Authors - Akbar Ahmad