TTBK2 Polyklonaler Antikörper

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• KD/KO Validated

TTBK2 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

Rezensionen (1)

Kat-Nr. : 15072-1-AP

Synonyme

TTBK, Tau-tubulin kinase 2, tau tubulin kinase 2, SCA11, KIAA0847

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  WB analysis of PC-3 using 15072-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 15072-1-AP (TTBK2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15072-1-AP

  WB result of TTBK2 antibody (15072-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TTBK2 transfected HeLa cells.

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  WB analysis of SH-SY5Y using 15072-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15072-1-AP (TTBK2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 15072-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15072-1-AP (TTBK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 15072-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15072-1-AP (TTBK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 15072-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15072-1-AP (TTBK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 15072-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15072-1-AP (TTBK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of hTERT-RPE1 using 15072-1-AP

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using TTBK2 antibody (15072-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CoraLite®594 ARL13B antibody (CL594-17711, red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen, HeLa-Zellen, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: hTERT-RPE1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 6 publications below

Produktinformation

15072-1-AP bindet in WB, IHC, IF/ICC, ELISA TTBK2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TTBK2 fusion protein Ag7108
• Vollständiger Name: tau tubulin kinase 2
• Berechnetes Molekulargewicht: 137 kDa
• Beobachtetes Molekulargewicht: 137-150 kDa
• GenBank-Zugangsnummer: BC071556
• Gene symbol: TTBK2
• Gene ID (NCBI): 146057
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TTBK2(tau tubulin kinase 2) plays an important role in the tau cascade and in spinocerebellar degeneration. The N-terminus of TTBK2 has a serine-threonine-tyrosine kinase domain, and a C-terminal region shows homology to TTBK1. TTBK1 and TTBK2 function as regulators of TDP-43 phosphorylation. TTBK1/2 may be attractive drug targets for therapeutic interventions in TDP-43 proteinopathies such as FTLD-TDP and ALS.(PMID:25473830). 15072-1-AP has a cross reaction with TTBK1.

Publikationen

Species	Application	Title
mouse	WB,IF	Cell Res; NudCL2 is an autophagy receptor that mediates selective autophagic degradation of CP110 at mother centrioles to promote ciliogenesis.; Authors - Min Liu
human	WB	PLoS Genet; The Tau Tubulin Kinases TTBK1/2 Promote Accumulation of Pathological TDP-43.; Authors - Nicole F Liachko
mouse	WB	EMBO Rep; XIAP-mediated degradation of IFT88 disrupts HSC cilia to stimulate HSC activation and liver fibrosis; Authors - Renjie Hong
human	IF	Sci Rep; MCRS1 associates with cytoplasmic dynein and mediates pericentrosomal material recruitment.; Authors - Si-Hyung Lee
human	IF	Hum Mol Genet; Altered gene regulation as a candidate mechanism by which ciliopathy gene SDCCAG8 contributes to schizophrenia and cognitive function.; Authors - Mairéad Flynn
human	IF	J Cell Sci; Differential requirements for the EF-hands of human centrin2 in primary ciliogenesis and nucleotide excision repair.; Authors - Ebtissal M Khouj

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Christine (Verified Customer) (01-27-2023)

Used on lysates from HEK293T cells. Incubation for a few hours at room temperature. Detection of 2 main bands, 1 above 118 kDa marker (expected size of TTBK2 is 137-150 kDa) and 1 between 47 and 85 kDa markers.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T