TTBK2 Polyklonaler Antikörper

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• KD/KO Validated

TTBK2 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15072-1-PBS

Synonyme

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• 

  WB analysis of PC-3 using 15072-1-AP (same clone as 15072-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 15072-1-AP (TTBK2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15072-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 15072-1-AP (same clone as 15072-1-PBS)

  WB result of TTBK2 antibody (15072-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TTBK2 transfected HeLa cells. This data was developed using the same antibody clone with 15072-1-PBS in a different storage buffer formulation.

• 

  WB analysis of SH-SY5Y using 15072-1-AP (same clone as 15072-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15072-1-AP (TTBK2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15072-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 15072-1-AP (same clone as 15072-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15072-1-AP (TTBK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15072-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 15072-1-AP (same clone as 15072-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15072-1-AP (TTBK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15072-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 15072-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15072-1-AP (TTBK2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 15072-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15072-1-AP (TTBK2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 15072-1-AP (same clone as 15072-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15072-1-AP (TTBK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15072-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 15072-1-AP (same clone as 15072-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15072-1-AP (TTBK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15072-1-PBS in a different storage buffer formulation.

• 

  IF Staining of hTERT-RPE1 using 15072-1-AP (same clone as 15072-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using TTBK2 antibody (15072-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 ARL13B antibody (CL594-17711, red). This data was developed using the same antibody clone with 15072-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

15072-1-PBS bindet in WB, IHC, IF/ICC, ELISA TTBK2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TTBK2 fusion protein Ag7108
• Vollständiger Name: tau tubulin kinase 2
• Berechnetes Molekulargewicht: 137 kDa
• Beobachtetes Molekulargewicht: 137-150 kDa
• GenBank-Zugangsnummer: BC071556
• Gene symbol: TTBK2
• Gene ID (NCBI): 146057
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TTBK2(tau tubulin kinase 2) plays an important role in the tau cascade and in spinocerebellar degeneration. The N-terminus of TTBK2 has a serine-threonine-tyrosine kinase domain, and a C-terminal region shows homology to TTBK1. TTBK1 and TTBK2 function as regulators of TDP-43 phosphorylation. TTBK1/2 may be attractive drug targets for therapeutic interventions in TDP-43 proteinopathies such as FTLD-TDP and ALS.(PMID:25473830). 15072-1-AP has a cross reaction with TTBK1.