TTC1 Polyklonaler Antikörper

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• KD/KO Validated

TTC1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 11676-1-AP

Synonyme

TPR1, TPR repeat protein 1

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  WB analysis of HEK-293 using 11676-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11676-1-AP (TTC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 11676-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11676-1-AP (TTC1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11676-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11676-1-AP (TTC1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11676-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11676-1-AP (TTC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11676-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11676-1-AP (TTC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 11676-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11676-1-AP (TTC1 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HepG2 using 11676-1-AP

  Immunofluorescent analysis of HepG2 cells using 11676-1-AP (TTC1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 11676-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TTC1 antibody (11676-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IP: See 1 publications below

Produktinformation

11676-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA TTC1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TTC1 fusion protein Ag2277
• Vollständiger Name: tetratricopeptide repeat domain 1
• Berechnetes Molekulargewicht: 33.5 kDa
• Beobachtetes Molekulargewicht: 34 kDa
• GenBank-Zugangsnummer: BC000942
• Gene symbol: TTC1
• Gene ID (NCBI): 7265
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB,IP	Mol Cell; Triaging of α-helical proteins to the mitochondrial outer membrane by distinct chaperone machinery based on substrate topology; Authors - Gayathri Muthukumar ; KD Validated