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TTC28 Polyklonaler Antikörper

TTC28 Polyklonal Antikörper für WB, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 25701-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of various lysates using TTC28 Polyclonal antibody (25701-1-AP)

    WB analysis using 25701-1-AP (same clone as 25701-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25701-1-AP (TTC28 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25701-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using TTC28 Polyclonal antibody (25701-1-AP)

    WB analysis of HeLa using 25701-1-AP (same clone as 25701-1-PBS)

    WB result of TTC28 antibody (25701-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TTC28 transfected HeLa cells. This data was developed using the same antibody clone with 25701-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using TTC28 Polyclonal antibody (25701-1-AP)

    IP experiment of HEK-293 using 25701-1-AP (same clone as 25701-1-PBS)

    IP result of anti-TTC28 (IP:25701-1-AP, 4ug; Detection:25701-1-AP 1:2500) with HEK-293 cells lysate 1480 ug. This data was developed using the same antibody clone with 25701-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

25701-1-PBS bindet in WB, IP, Indirect ELISA TTC28 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen TTC28 fusion protein Ag22465
Vollständiger Name tetratricopeptide repeat domain 28
Berechnetes Molekulargewicht 2481 aa, 271 kDa
Beobachtetes Molekulargewicht240-271 kDa
GenBank-ZugangsnummerBC016465
Gene symbol TTC28
Gene ID (NCBI) 23331
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TTC28 is a large protein required during the cell cycle for condensation of spindle midzone microtubules, formation of the midbody, and completion of cytokinesis. RT-PCR ELISA detected highest TTC28 expression in adult ovary and lung, followed by kidney and liver. Lower expression was detected in all other adult and fetal tissues examined except testis, which showed no expression.