TTC35 Polyklonaler Antikörper

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• KD/KO Validated

TTC35 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(15)

Kat-Nr. : 25443-1-AP

Synonyme

EMC2, ER membrane protein complex subunit 2, KIAA0103, TPR repeat protein 35

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  WB analysis of Jurkat using 25443-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 25443-1-AP (TTC35 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 25443-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25443-1-AP (TTC35 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of SKOV-3 using 25443-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 25443-1-AP (TTC35 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 25443-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 25443-1-AP (TTC35 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 25443-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 25443-1-AP (TTC35 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 25443-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 25443-1-AP (TTC35 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 25443-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 25443-1-AP (TTC35 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 25443-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 25443-1-AP (TTC35 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 25443-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 25443-1-AP (TTC35 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 25443-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 25443-1-AP (TTC35 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 25443-1-AP

  IP result of anti-TTC35 (IP:25443-1-AP, 3ug; Detection:25443-1-AP 1:600) with mouse heart tissue lysate 4000ug.

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  IHC staining of human ovary cancer using 25443-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 25443-1-AP (TTC35 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, humanes Plazenta-Gewebe, K-562-Zellen, MCF-7-Zellen, Mausherzgewebe, Mausmilzgewebe, NIH/3T3-Zellen, PC-3-Zellen, SKOV-3-Zellen
• Erfolgreiche IP: Mausherzgewebe
• Erfolgreiche Detektion in IHC: human ovary cancer tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 14 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

25443-1-AP bindet in WB, IHC, IF, IP, ELISA TTC35 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TTC35 fusion protein Ag22196
• Vollständiger Name: tetratricopeptide repeat domain 35
• Berechnetes Molekulargewicht: 297 aa, 35 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC021667
• Gene symbol: TTC35
• Gene ID (NCBI): 9694
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TPR repeat protein 35 (TTC35), also named as tetratricopeptide repeat domain 35, is a 297 amino acid protein, which contains three TPR repeats and belongs to the EMC2 family. TTC35 localizes in the nucleus and cytoplasm. TTC35 is a component of the ER membrane protein complex and may be a putative O-linked glycosyl transferase.

Publikationen

Species	Application	Title
human	WB	Cell; EMC Is Required to Initiate Accurate Membrane Protein Topogenesis.; Authors - Patrick J Chitwood
human	WB	Mol Cell; WNK1 is an assembly factor for the human ER membrane protein complex.; Authors - Tino Pleiner
human	WB	EMBO Mol Med; SLC25A46 is required for mitochondrial lipid homeostasis and cristae maintenance and is responsible for Leigh syndrome.; Authors - Alexandre Janer
human	WB	J Cell Biol; A selectivity filter in the ER membrane protein complex limits protein misinsertion at the ER; Authors - Tino Pleiner
human	WB	Elife; The ER membrane protein complex is required to ensure correct topology and stable expression of flavivirus polyproteins.; Authors - Ashley M Ngo
human	WB	Elife; Structural and mechanistic basis of the EMC-dependent biogenesis of distinct transmembrane clients.; Authors - Lakshmi E Miller-Vedam ; KO Validated