TTC37 Polyklonaler Antikörper

TTC37 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 24594-1-AP

Synonyme

TPR repeat protein 37, Thespin, Superkiller complex protein 3, SKIC3, Ski3

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  WB analysis using 24594-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24594-1-AP (TTC37 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 24594-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 24594-1-AP (TTC37 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 24594-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24594-1-AP (TTC37 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 24594-1-AP

  IP result of anti-TTC37 (IP:24594-1-AP, 4ug; Detection:24594-1-AP 1:300) with mouse testis tissue lysate 4000ug.

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  IHC staining of human kidney using 24594-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24594-1-AP (TTC37 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human tonsillitis using 24594-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24594-1-AP (TTC37 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 24594-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24594-1-AP (TTC37 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human tonsillitis using 24594-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24594-1-AP (TTC37 Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of A549 using 24594-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using TTC37 antibody (24594-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, MCF-7-Zellen, Maushodengewebe
• Erfolgreiche IP: Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below

Produktinformation

24594-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA TTC37 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TTC37 fusion protein Ag18442
• Vollständiger Name: tetratricopeptide repeat domain 37
• Berechnetes Molekulargewicht: 1564 aa, 175 kDa
• Beobachtetes Molekulargewicht: 140-150 kDa
• GenBank-Zugangsnummer: BC056893
• Gene symbol: TTC37
• Gene ID (NCBI): 9652
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TTC37 (tetratricopeptide repeat protein 37), also named as Thespin, encodes 1,564 amino acid protein that contains twenty tetratricopeptide (TPR) repeats. TPR repeat-containing motifs are found in a variety of proteins and may mediate protein-protein interactions and chaperone activity. Although not expressed in liver, TTC37 is a widely expressed protein with highest levels of expression in vascular tissues, lymph node, pituitary, lung and intestine. TTC37 is a component of the SKI complex which is thought to be involved in exosome-mediated RNA decay and associates with transcriptionally active genes in a manner dependent on PAF1 complex (PAF1C). Defects in TTC37 are associated with Trico-Hepato-Enteric (THE) syndrome.

Publikationen

Species	Application	Title
human,mouse	WB	J Clin Invest; Cytoplasmic RNA quality control failure engages mTORC1-mediated autoinflammatory disease.; Authors - Kun Yang
human	WB	PLoS Genet; A Comprehensive Genomic Analysis Reveals the Genetic Landscape of Mitochondrial Respiratory Chain Complex Deficiencies.; Authors - Masakazu Kohda
human	WB	Mol Cancer Ther; Targeting the synthetic lethal relationship between FOCAD and TUT7 represents a potential therapeutic opportunity for TUT4/7 small molecule inhibitors in cancer; Authors - Robinson Triboulet
human	WB	EMBO J; RNA helicase SKIV2L limits antiviral defense and autoinflammation elicited by the OAS-RNase L pathway; Authors - Kun Yang ; KO Validated