TTC4 Polyklonaler Antikörper

TTC4 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Rezensionen (1)

Kat-Nr. : 11878-1-AP

Synonyme

TPR repeat protein 4, TTC4

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• 

  WB analysis of HeLa using 11878-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11878-1-AP (TTC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11878-1-AP

  WB result of TTC4 antibody (11878-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TTC4 transfected HEK-293 cells.

• 

  WB analysis of HEK-293 using 11878-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11878-1-AP (TTC4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11878-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11878-1-AP (TTC4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lymphoma using 11878-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 11878-1-AP (TTC4 antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Lymphomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

11878-1-AP bindet in WB, IHC, ELISA TTC4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TTC4 fusion protein Ag2474
• Vollständiger Name: tetratricopeptide repeat domain 4
• Berechnetes Molekulargewicht: 387 aa, 45 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC001276
• Gene symbol: TTC4
• Gene ID (NCBI): 7268
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Coffee (Verified Customer) (03-20-2022)

Verified by knockdown at expected size, but there is an additional non-specific band underneath it.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: K562 cell

TTC4 Antibody Western Blot validation (1:1000 dilution) in K562 cell (Cat no:11878-1-AP)